福莫特罗和ICI118551对大鼠成熟破骨细胞骨吸收功能的影响

目的研究选择性β2肾上腺素能激动剂福莫特罗(Formoterol)和阻滞剂ICI118551对体外培养大鼠成熟破骨细胞(osteoclast,OC)功能的影响,探讨β2肾上腺素能受体信号对骨代谢的影响。方法取清洁级出生24h内的SD乳大鼠,长骨干骨髓腔内壁机械分离成熟OC后分别加入不同浓度(10-5mol/L～10-9mol/L)的Formoterol和ICI118551,以抗酒石酸酸性磷酸酶(TRAP)染色观察破骨细胞形态,甲苯胺蓝染色计数骨片上的骨吸收陷窝数目,Image-ProPlus6.0图像软件分析骨片上骨吸收陷窝面积。结果破骨细胞与骨片共培养6天,不同浓度的Formoterol与对照组相比均可增加骨片上OC的骨吸收陷窝数目和面积;随着ICI118551浓度的提高骨片上骨吸收陷窝的数目和面积逐渐减少。结论β2肾上腺素能受体激动剂可促进体外培养OC的骨吸收功能,阻滞剂对OC的骨吸收功能有抑制作用,且呈剂量依赖性。

The effects of Formoterol and ICI118551 on rat osteoclast in vitro

Objective To evaluate the effects of Formoterol(β_2-adrenergic receptor-specific agonist) and ICI118551 (β_2-adrenergic receptor-specific antagonist) on rat matured ostoeclast cells in vitro.Methods Mature OC was isolated directly from neonatal 24-hour-SD rat long bones.The OC was treated with different concentrations of Formoterol and ICI118551 from 10~(-5) mol/L～10~(-9) mol/L respectively.Then the OC was stained with tartrate-resistant acid phosphatase (TRAP) to observe cellular morphology.The bone slices were stained with toluidine blue to count the number of the bone resorption pits and analyse the bone resorption pits by Image-Pro Plus 6.0.Results The number and area of the bone resorption pits increased in different concentrations of Formoterol treatment compared that in control treatment after osteoclasts and bone slices co-cultured for 6 days.Furthermore,the number and area of the bone resorption pits decreased with the dose of ICI118551 increased gradually.Conclusion The β_2-adrenergic receptor-specific agonist could promote the bone absorbion of OC in vitro.The β_2-adrenergic receptor-specific antagonist inhibits the bone resorption of OC in a dose-dependent manner.