大鼠脑局灶缺血／再灌注对外周血单个核CD34^＋细胞的影响

目的本研究探讨大鼠脑局灶缺血/再灌注是否影响外周血单个核CD34+细胞的数量.方法采用大鼠大脑中动脉(MCA)缺血/再灌注线栓法模型,将大鼠随机分为3组对照组,假手术组,动物模型组.取再灌注后1、3、6、12 h和1、2、3、4、7、14 d,10个观察点,测外周血单个核CD34+表达情况.结果大鼠脑缺血/再灌注模型中外周血单个核CD34+细胞在再灌注后1 h～2 d无明显变化,在脑缺血/再灌注后3～7 d明显减少,14 d后恢复.结论大鼠脑缺血/再灌注模型再灌注后1 h～2 d再灌注后外周血单个核CD34+细胞没有变化,在脑缺血/再灌注后3～7 d明显减少.

Impact of Focal Cerebral Ischemia/Reperfusion on Circulating CD34+ Cells in Rat

Aim:This study was intended to detect changes of circulating CD34 + cells after focal cerebral ischemia/reperfusion in rats.Methods:A model of a suture occlusion of right middle cerebral artery (MCA) of male SD ratd was used.60 mature male SD rats were randomly divided into 12 groups:control group,sham-operated control group,1-hour (1h),3h,6h,12h,24h,2-day (2d),3d,4d,7d,,14d reperfusion groups.After right middle cerebral artery occlude,the rats were suffered from ischemia for 2 hours, then reperfusion was allowed for different time as mentioned above before decapitation.Circulating CD34 + cells were detected by cytoflowmetric analysis.Results:There was no influence on circulating CD34 + cells in rat MCAO models from 1h to 2 days after reperfusion.But circulating CD34 + cells sharply decreased from 3 to 7 days after reperfusion and returned to basal values at 14th day after cerebral reperfusion.Conclusion:Circulating CD34 + cells sharply decreased from 3 to 7 days after reperfusion in rat MCAO models.