| 抗人结肠癌单克隆抗体细胞株的建立及其免疫组化研究 |
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| 引用本文： | 施作榕,王振明,吴轰,冯铁剑,卜湘,魏启幼,周小燕. 抗人结肠癌单克隆抗体细胞株的建立及其免疫组化研究[J]. 中南大学学报(医学版), 1986, 0(4) |
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| 作者姓名： | 施作榕 王振明 吴轰 冯铁剑 卜湘 魏启幼 周小燕 |
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| 作者单位： | 湖南医学院第二附属医院中心实验室(施作榕，王振明，吴轰，冯铁剑，卜湘，魏启幼)，湖南医学院第二附属医院中心实验室(周小燕) |
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| 摘 要： | 本文报道了抗人结肠癌单克隆抗体3E_4细胞株的建立方法。其上清液与各种人癌细胞株及正常组织细胞等反应均阴性,仅与AFP似有弱交叉反应;经免疫组化研究,3E_4株单克隆抗体对结肠癌粘膜组织有一定选择性,但与癌旁正常结肠组织仍有一定交叉反应,属于抗结肠癌相关抗原的单克隆抗体。
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| 关 键 词： | 单克隆抗体 结肠肿瘤 免疫化学 组织细胞化学 |
PRODUCTON OF MONOCLONAL ANTI-HUMAN COLOR-ECTAL CARCINOMA CELL STRAIN AND EVALUTION WITH IMMUNDHISTOCHEMISTRY STUDY |
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| Shi Zuoyong Wang Zhenming Wu Hong Bu Xiang Feng Tiejian Wei Qiyou Zhou XiaoyanCentral Lab The Second Affiliated Hospital,Hunan Medical College. PRODUCTON OF MONOCLONAL ANTI-HUMAN COLOR-ECTAL CARCINOMA CELL STRAIN AND EVALUTION WITH IMMUNDHISTOCHEMISTRY STUDY[J]. Journal of Central South University. Medical sciences, 1986, 0(4) |
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| Authors: | Shi Zuoyong Wang Zhenming Wu Hong Bu Xiang Feng Tiejian Wei Qiyou Zhou XiaoyanCentral Lab The Second Affiliated Hospital Hunan Medical College |
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| Abstract: | 3E4 hybridoma cell strain was produced by hybridoma derived from the fusion of mouse myeloma cells with splenocytes of HRT-18 human colorectal carcinoma cell strain immunized Babl/c mice, which secreted colorectal carcinoma antibodies(McAb). The McAb had been identified by immunofluorescence and immunity histochemisry and showed that McAb was provided with selectivity for human colorectal carcinoma, but did weakly react with the patient's morphologically normal colon mucosa. It is suggested that monoclonal anti-human colorectal carcinoma anfibody secreted by 3E4 cells is selecfive. |
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| Keywords: | Immunochemistry Antibodies monoclonal Histocytochemistry Colon neoplasms |
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