口腔扁平苔藓患者白色念珠菌分离株的基因型分析

目的 研究健康人和口腔扁平苔藓(oral lichen planus，OLP)患者口腔白色念珠菌分离株的基因型分布特征．方法 应用随机扩增片段多态性分析法(randomly amplified polymorphic DNA，RAPD)，对来自于健康人(26株)、糜烂型OLP患者(62株)以及非糜烂型OLP患者(24株)共112株白色念珠菌菌株做基因型分型，比较OLP患者与健康对照组白色念珠菌分离株的基因型分布差异．结果 本研究采用的RAPD分析法可大致将所有白色念珠菌分离株分为A、B、C、D四种基因型；健康对照组、糜烂型OLP和非糜烂型OLP患者组的白色念珠菌分离株的基因型构成具有差异，其中，健康对照组以B、D型为主，糜烂型OLP患者组以A、C型为主，非糜烂型OLP患者组以A、D型为主。结论 某些具有特定基因型的白色念珠菌与OLP的发生发展可能相关。

The Genotypic Profiles of Candida albicans Isolates from Patients with Oral Lichen Planus

Objective The aim of this study was to investigate the genotypic profiles of Candida albicans isolates from erosive oral lichen planus(OLP) and non-erosive OLP. Methods A total of 112 isolates obtained from healthy control (26), erosive OLP (62) and non-erosive OLP (24) were screened for genotypic profiles by using the randomly amplified polymorphic DNA (RAPD) assay. Results RAPD analyses with some random primer reavealed 4 different genotypes among all isolates, and there was significant difference in genotypic constitution between every two groups. Statistically, B and D were the major types in healthy group; A and C were the major types in erosive OLP; A and D were the major types in non-erosive OLP. Conclusion Some Candida albicans isolates with special genotypic profiles may contribute to the development and progression of OLP.