中药谱效学研究方向方法初探

目的：提出中药谱效学基本概念、研究方向及方法。方法：根据目前中医药学科的现代科学属性,结合相关交叉学科知识与文献,以及近几年科研工作,对其方法进行初探。结果：提出了首先应建立与动物(人)“证”模型相对应的状态函数关系式的现代中医药数理表述体系,再根据与“证”相一致所对应的基因表达下特性蛋白质与效应体(药物)的齿合关系,按亲合色谱,以效应体靶向分离物——特性蛋白质为固定相,采用LC／MS质量或效应型检测器,建立质量或效应指纹图谱。根据效应体作用前后(体内药物浓度为零)效应值的变化趋势研究该指纹图谱与生物体状态函数值变化关系,可揭示中药复方作用物质基础：包括效应体(结合成分群)的数目及构成比,以及对机体的作用方向和程度。结论：中药谱效学研究为中医药体系现代化的关键问题,我们应稳妥推动中药谱效学的建立和发展。

Preliminary Exploration of Orientation and Method of Chromatographic Pharmacodynamics of Traditional Chinese medicine

Objective: To propose the basic concepts and the study orientation and method of the chromatographic pharmaco-dynamics of traditional Chinese medicine (TCM). Method: To preliminarily explore its study method in accordance with the modern characters of TCM disciplines and in combination of the knowledge and literature of relevant cross - disciplines as well as the research results made in recent years. Result: It is proposed that a system of TCM mathematic expression of state function relationship, which corresponds to the " syndromes" of animal ( human being ) models and then a fingerprint of quality or efficiency should be set up according to the relationship of indented joint between specific proteins and effectors ( drugs ) under gene expression corresponding to the " syndromes", with the target separators of effectors-the specific proteins based on affinity chromatograph as its stationary phase and by the application of LC/MS for the detection of its quality and efficiency. As the result the fundamentals of the functional matters of TCM compounds, including the number and component ratio of the effectors as well as the functional direction and degree of body can afford to be brought to light by studying the relationahip of the change between the fingerprint and the state function value of organisms in accordance with the changing tendancy of the efficiency value before and after the functioning of effectors ( when the concentration of drugs in vivo is zero ) . Conclusion: The study of TCM chromatographic phar-macodynamics is a key to the modernization of TCM system and therefore the establishment and development of it should be steadily pushed forward.