大鼠脑外伤后P-selectin表达的变化及意义

目的 探讨大鼠脑外伤后P-selectin的表达变化及其与脑水肿等继发性脑损伤的关系。方法 采用Feeney大鼠脑损伤动物模型，将60只Sprague-Dawley大鼠随机分为3组：A（标准对照组），B（轻伤组）、C组（重伤组），每组20只。分别于脑外伤后6h、24h、3d、7d随机各取标本5只，测量脑水肿程度以及P-selectin在脑组织的表达变化以及免疫阳性染色血管，并进行计算机图像分析系统和统计学分析。结果 外伤后6h P-selectin的表达及粒细胞在脑组织的浸润显著升高（P〈0．05），于伤后24h达到高峰，然后逐渐降低。结论 脑外伤后P-selectin的表达及粒细胞在脑组织的浸润显著升高，其时程和空间分布与脑外伤后继发性损伤相吻合。提示P-selectin的表达增加可能参与了脑外伤后的继发性损伤。

Changes in P-selectin expression after brain injury in rats

Objective To explore the relationship between expression thange of P-selectin after brain injury and secondary brain damage.Methods Sixty SD rats were randomized into 3 equal groups,namely the control group,mild injury group and severe injury group and animal models of brain injury were established in SD rats according to the method of Feeney.P-selectin expression in the brain tissues were determined at 6 h and l,3,and 7 days following brain injury(n=5 for each time point).Imaging analysis was performed using computerized imaging technique.Results P-selectin expression and neutrophil infiltration in the brain tissues increased significantly 6 h after brain injury(P<0.05),reaching the peak level at postoperative 24 h and then gradually decreased.Conclusion P-selectin expression and neutrophil infiltration increase significantly following brain injury,and the time course and distribution of P-selectin expression are consistent with the secondary damage of the brain,strongly suggesting the involvement of P-selectin upregulation in the secondary insult after brain injury.