A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I–V |
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| Authors: | K. Boye,M. D. Bartels,I. S. Andersen,J. A. Mø ller, H. Westh |
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| Affiliation: | Department of Clinical Microbiology, Hvidovre Hospital, Hvidovre, Denmark. Kit.Boye@hvh.regionh.dk |
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| Abstract: | A multiplex PCR with four primer-pairs was designed to identify the five main known SCCmec types. A clear and easily discriminated band pattern was obtained for all five types. The SCCmec type was identified for 98% of 312 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). SCCmec type IV was by far the most common SCCmec type among both hospital- and community-acquired MRSA isolates in Denmark. |
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| Keywords: | Identification molecular MRSA multiplex PCR SCCmec type Staphylococcus aureus |
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