正常儿童血清中邻苯二甲酸二丁酯和邻苯二甲酸-2-乙基己酯水平的测定

目的检测正常儿童血清中邻苯二甲酸二丁酯(DBP)和邻苯二甲酸-2-乙基己酯(DEHP)水平。方法选择上海市正常儿童作为研究对象,用气相色谱检测其血清中DBP、DEHP含量,作正常儿童血清DBP、DEHP浓度分布,以及DBP、DEHP不同年龄和性别水平分析。结果 310例血样中,110例可检出DBP,118可检出DEHP,全部样品中DBP的浓度范围为未检出～0.98ng/ml,P75为0.68ng/ml,95%上限为0.75ng/ml,男女检出率无差别,检出率在不同年龄组间存在差别。全部样品中DEHP的浓度范围为未检出～0.94ng/ml,P75为0.67ng/ml,95%上限为0.74ng/ml,男女检出率无差别,检出率在不同年龄组间存在差别,婴儿组的检出率明显低于其他组。结论正常儿童的血清DBP、DEHP浓度较低,DBP低于0.75ng/ml,DEHP低于0.74ng/ml。

Dectective of serum di-n-butyl phthalate and di-2-ethylhexyl phthalate level in normal children

Objective To study on the level of serum di-n-butyl phthalate( DBP),di-2-ethylhexyl phthalate (DEHP) in normal children by using gas chromatography.Methods Gas chromatography method was established.DBP,DEHP in normal children were detected ,and their reference level was calculated,DBP and DEHP level in different age and genders.were analysed Results DBP could be detected in 110 serum samples from total 310 samples,and DEHP could be detected in 118 serum samples from the total 310 samples.The detected contrations of DBP range from non detectable to 0.98ng /ml,P75 was 0.68ng /ml,P95 was 0.75ng /ml.There was no difference in the detection ratios between females and males,while baby group detection ratios was lower than other groups..The detected contrations of DEHP range from non detectable to 0.94ng /ml,P75 was 0.67ng /ml,P95 was 0.74ng /ml.There was no difference in the detection ratios between females and males.While there was difference among age groups.Conclusion The study domonstrated that DBP and DEHP concentrations in normal children are very low.The refrence level of DBP was suggested as lower than 0.75ng /ml,DHEP was suggested as lower than 0.74ng /ml.