缺氧诱导因子1α及凋亡相关蛋白Survivin、Bax在肺非小细胞肺癌中的表达及其临床意义

目的探讨缺氧诱导因子1α（HIF-1α）在肺非小细胞肺癌（NSCLC）中的表达及与凋亡相关蛋白Survivin、Bax表达的关系。方法采用免疫组织化学方法检测52例NSCLC组织中HIF-1α和Survivin、Bax的表达。结果NSCLC组织中HIF-1α、Survivin、Bax的阳性表达率分别为38．5％（20／52）、48．1％（25／52）和42．3％（22／52）。HIF-1α的表达与肿瘤临床分期（x^2=8．271,P〈0．05）及淋巴结转移（x^2=6．370,P〈0．05）相关,与肿瘤病理类型（x^2=0．561,P〉0．05）及分化程度（x^2=0．326,P〉0．05）无明显相关性。HIF-1α的表达与Survivin表达（1=0．496,P〈0．05）和Bax表达（γ=0．681,P〈0．05）正相关。结论HIF-1α表达与NSCLC的浸润性发展、转移及凋亡发生密切相关。

Expression of hypoxia inducible factor-1alpha and relationship thereof to apoptosis related protein in non-small cell lung cancer

OBJECTIVE: To investigate the expression of hypoxia inducible factor-1alpha (HIF-1alpha) and relationship thereof to survivin and Bax, apoptosis related proteins, in non-small cell lung cancer (NSCLC). METHODS: Immunohistochemistry was used in 52 specimens of NSCLC to detect the expression of HIF-1alpha, survivin and Bax. RESULTS: The positive rates of HIF-1alpha, survivin and Bax were 38.5% (20/52), 48.1% (25/52), and 42.3% (22/52) respectively. The higher the pathological stage of tumor, the higher expression rate of HIF-1alpha (chi2=8.271, P<0.05). The positive rate of HIF-1alpha expression of the patients with lymph node metastasis was higher than that of the patients without lymph node metastasis (chi2=6.370, P<0.05). However, HIF-1alpha expression had no relationship to the pathological type (chi2=0.561, P>0.05) and differentiation degree (chi2=0.326, P>0.05). Correlation analysis showed that the expression of HIF-1alpha was positively correlated with the surviving expression (gamma=0.496, P<0.05) and Bax expression (gamma=0.681, P<0.05). CONCLUSION: The expression of HIF-1alpha is related to the infiltration, metastasis, and apoptosis of NSCLC.