COX-2蛋白在鼻咽癌中的表达及其临床意义

目的探讨COX-2（cyclooxygenase-2）蛋白在鼻咽癌组织中的表达及其临床意义。方法分别应用免疫组化方法（nac）和蛋白印迹方法（WesternBlot）检测47例鼻咽癌组织（其中用IHC33例，Western Blotl 4例）及24例鼻咽部炎症组织中COX-2蛋白的表达。结果COX-2蛋白在鼻咽癌组织的阳性表达率为81．8％（27／33），在鼻咽炎组织的阳性表达率为25％（3／12），两者表达差异有统计学意义（P=0．001）。WesternBlot结果显示，在两种组织中COX-2蛋白表达的相对值分别为1．258±0．034和0．487±0．076，在鼻咽癌组表达显著增高（P=0．000）。结论COX-2蛋白的高度表达在鼻咽癌的发生、发展中起重要作用，选择性COX-2抑制剂有望成为鼻咽癌化疗的重要药物。

Expression and clinical significance of cox-2 protein in nasopharyngeal carcinoma

Objective To investigate expression and its clinical significance of COX-2 protein in nasopharyngeal carcinoma. Methods The inamunohistochemical method and Western blot method were used to determine expression of COX-2 protein in specimens of 47 cases （33 cases by IHC and 14 by Western Blot ） of nasopharyngeal carcinoma and 24 cases of normal nasopharyngitis tissues. Results Positive staining rate of COX-2 in specimens of nasopharyngeal carcinoma （81.8 % ） was significantly higher than that of normal nasopharyngitis tissues （25 % ） （P = 0.001 ）. Western Blot assay revealed that the relative COX-2 protein level was 1. 258 ± 0.034 and 0. 487 ± 0.076 respectively in the two tissues,which was significantly higher in nasopharyngeal carcinoma （P = 0. 000 ）. Conclusion Over-expression of COX-2 may play a crucial role in the carcinogenesis and progression of nasopharyngeal carcinoma, selectivity COX-2 inhibiter may become an important chemotherapy drug for nasopharyngeal carcinoma.