全反式维甲酸对荷瘤Wistar大鼠生存期的影响及其机制

 目的研究全反式维甲酸对荷瘤鼠生存期的影响及其机制。方法建立wistar大鼠脑内C6胶质瘤模型，待肿瘤生长至第7天，随机将荷瘤鼠分为维甲酸治疗组和对照组。于接种后的第22天，免疫组化检测各组P27Kip1蛋白的表达并观察动物生存期。结果与对照组相比，治疗组动物p27kip1蛋白的表达增高（P〈0．05），生存期延长（P〈0．05），两者呈正相关。结论全反式维甲酸延长动物生存期的机制同上调p27kip1蛋白的表达有关。p27kip1蛋白的表达高低可作为判断胶质瘤预后的指标。

Effect of Matrine on Signaling Transduction of MAPK and JAK-STAT in SMMC-7721 Cell Line

Objective 　To investigate the effect of mat rine on MAPK and JA K2STAT signaling t ransduc2 tion pathway in SMMC27721 cell line. Methods 　Treated with mat rine and A G490 (the inhibitor of JA K2 STAT signaling t ransduction) , the mRNA expression of ERK, STAT3 and STAT5 in SMMC27721 cell line were assessed with RT2PCR. The protein expression of ERK, STAT3 , STAT5 , P2ERK, P2STAT3 and P2STAT5 in SMMC27721 cell line were detected by Western blotting. Results 　The mRNA expres2 sion of ERK, STAT3 and STAT5 in SMMC27721 cell with mat rine was significantly lower than those in cont rol group ( P < 0. 05 or P < 0. 01) and so did the protein expression of ERK, STAT3 , STAT5 , P2 ERK, P2STAT3 and P2 STAT5 ( P < 0. 05 or P < 0. 01) . There were no significant difference of the mR2 NA expression of ERK, STAT3 and STAT5 between A G490 and cont rol group ( P > 0. 05) , and so did the protein expression of ERK, STAT3 and STAT5 ( P > 0. 05) . But P2ERK, P2STAT3 and P2 STAT5 protein were significantly lower than those in cont rol group ( P < 0. 05 or P < 0. 01) . After t reated with mat rine plus AG490 , the mRNA expression of ERK, STAT3 and STAT5 were significantly lower than those in AG490 group ( P < 0. 05) and so did the protein expression of ERK, STAT3 , STAT5 ( P < 0. 05) . There were no significant difference of the P2ERK, P2STAT3 and P2 STAT5 protein between mat rine plus A G490 group and A G490 group ( P > 0. 05) . There were no significant difference of the mRNA expression of ERK, STAT3 and STAT5 between mat rine plus A G490 group and mat rine group ( P > 0. 05) , and so did ERK, STAT3 , STAT5 , P2ERK, P2STAT3 and P2 STAT5 protein ( P > 0. 05) . Conclusion 　Mat rine could significantly down2regulate the mRNA expression of ERK, STAT3 , STAT5 and protein expression of ERK, STAT3 and STAT5 in SMMC27721 cells. So mat rine could inhibit the signaling t ransduction pathway of MAPK and JA K2STAT and inhibit the proliferation of SMMC27721 cells.