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目的研究发育期SD大鼠髁状突颈部骨折对下颌髁状突软骨细胞中上游刺激因子1(USF1)表达的影响。方法本研究于2011年7—9月在昆明医科大学口腔医学院研究所完成。选择4周龄雄性SD大鼠12只,其中6只大鼠用于制作下颌单侧髁状突颈部骨折动物模型,6只大鼠为对照。分别在术后1、3、5周时处死大鼠,取出骨折侧、骨折对侧以及空白对照的髁状突软骨。采用免疫组织化学方法检测髁状突软骨细胞中USF1表达情况。结果术后1周,骨折对侧空白对照组与骨折侧的USF1表达有显著性差异(P<0.01);术后3周,空白对照组与骨折侧USF1表达有显著性差异(P<0.01)。骨折侧术后3周、5周与术后1周USF1表达有显著性差异(P<0.01)。结论单侧髁状突颈部骨折引起应力变化,使患侧髁状突软骨细胞中USF1表达异常,进而影响髁状突的生长发育。

Effect of unilateral condylar neck fracture on USF1 expression of the chondrocyte in growing rat

Objective To investigate the effect of unilateral condylar neck fracture on USF1 expression of the chondrocyte in growing rats. Methods Twelve 4-week-old rats were randomly divided into two parts： half were operated to cause condylar fracture, the other part as control. The rats were killed at 1,3 and 5 weeks after surgery, and the condylar cartilage was obtained from the operating side and non-operating side of experimental group and control group. Immunohistochemical staining was used to qualitively examine USF1 expression. Results There was significant difference between non-operating side, control group and operating side at 1 week after surgery. There was significant difference between operating side and control group at 3 weeks after surgery. There was significant difference between 3 and 5 weeks and 1 week after surgery on operating side. Conclusion The unbalanced stress caused by unilateral condylar neck fracture change USF expression affect the development and growth of condyle.