微量快速制备的人双核淋巴细胞微核标本及在放射生物学中的应用 |
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引用本文: | 余从年 周光云. 微量快速制备的人双核淋巴细胞微核标本及在放射生物学中的应用[J]. 癌变.畸变.突变, 1996, 8(4): 223-227 |
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作者姓名: | 余从年 周光云 |
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作者单位: | 同济医科大学生物教研室,德国埃森大学医学院医学放射生物学研究所 |
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摘 要: | 本文报道了利用细胞松弛素B抑制细胞质分裂诱导产生双核淋巴细胞的制备方法,该方法具有微量,快速,简便的优点,适用大范围的人群普查,具有一定的推广价值。并测试了10名健康献血者的外周血,经不同剂量x射线照射后的剂量反应曲线,其微核率照射剂量的增加而增加。
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关 键 词: | 微核 双核淋巴细胞 X射线 放射生物学 |
THE SPECIMEN OF HUMAN BINUCNEATE LYMPHOCYTES IN SMALL QUANTITY RAPIDLY PREPARATORY METHOD AND THE USE IN RADIATION BIOLOGY |
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Abstract: | This paper investigated that the utilization of cytochalasing B (CB) to inhibit cytoplasmic division and to produce binucneate lymphocytes.This method has micro,rapid and simple special advantage.It is fit for popular examination in sphere of crowd people.It is of definate promote value.We tested peripheral blood of ten healthy persons of donnate blood,the curve of response after irradiating with X-ray of different doses with which micronuclei rate increased,showed that this method may act as a sensitive biological dosimetry. |
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