新型隐球菌动物模型的建立及PCR快速诊断

目的建立从活检肺组织中进行隐球菌PCR诊断的小鼠模型。方法向BALB/c小鼠气管插管内注入隐球菌菌液制成小鼠隐球菌感染模型,取肺组织匀浆提取DNA进行PCR诊断。结果从位于新型隐球菌基因组的编码核糖体RNA(rRNA)和位于其中的内部转录区(internal transcribed spacer,ITS-1和ITS-2)基因位点的8对引物中筛选出1对引物(ITS1-CN4),其对从活检肺组织中诊断新型隐球菌具有较好的敏感性和特异性。结论成功建立了从活检肺组织中进行隐球菌PCR诊断的小鼠模型。从8对引物中筛选出的1对引物(ITS1-CN4)对从活检肺组织中诊断新型隐球菌具有较好的敏感性和特异性。

A Murine Model of Cryptococcus Neoformans Infection and PCR Rapid Diagnosis

Objective The authors established a cryptococcus neoformans-inoculated murine model which can be used to explore the diagnostic effect of cryptococcosis by PCR method. Methods The murine model for PCR diagnostic use was made by inoculating cryptococcus agents through tracheal intubation in BALB/c mice. PCR diagnosis was tested on DNA samples extracted from lung tissue of challenged mice. Results A pair of primers(ITS1-CN4) from eight pairs of candidate primers were screened out, which targeted the ribosomal DNA gene cluster and ITS1, ITS2 regions and could detect cryptococcus from murine tissue sensitively and specifically. Conclusion The murine model of cryptococcus neoformans infection were successfully established. One pair of primers(ITS1-CN4) could detect cryptococcus from murine tissue with high sensitivity and specificity.