| EGCG对鼻咽癌细胞株裸鼠移植瘤的放疗增敏作用以及对Survivin表达的影响 |
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| 引用本文： | 雷迅,刘强和,孔中雨,向秋,耿宛平,黄辉,董译元,刘芳贤. EGCG对鼻咽癌细胞株裸鼠移植瘤的放疗增敏作用以及对Survivin表达的影响[J]. 山东医大基础医学院学报, 2009, 0(1) |
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| 作者姓名： | 雷迅 刘强和 孔中雨 向秋 耿宛平 黄辉 董译元 刘芳贤 |
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| 作者单位： | 桂林医学院附属医院耳鼻咽喉-头颈外科;桂林医学院科学实验中心;桂林医学院附属医院肿瘤放疗科; |
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| 基金项目： | 广西科学基金资助项目(桂科自0640187);;广西医疗卫生重点课题(重200652) |
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| 摘 要： | 目的研究凋亡抑制基因Survivin的表达与EGCG对鼻咽癌CNE-2裸鼠移植瘤放疗增敏作用的关系。方法将低分化鼻咽癌细胞CNE-2接种于4~6周龄的雌性裸鼠皮下,待移植瘤生长至4~6 mm时,随机分为4组,分别采用NS、EGCG[50 mg/(kg.w)]、放疗(15 Gy)、EGCG给药24 h后再行放疗等方法分别处理各组裸鼠,处理前后分别测量裸鼠移植瘤的短径、体积。21 d后处死裸鼠,取出肿瘤并称重,将肿瘤组织分成两份,分别用来进行病理切片TUNEL染色以及RT-PCR检测Survivin mRNR的表达。结果EGCG+放疗对裸鼠移植瘤的生长抑制最为明显,肿瘤生长抑制率为89.3%,消退率为40.0%。与对照组EGCG组、放疗组相比,EGCG+放疗组的凋亡指数均明显增加(P<0.05),同时,伴有Survivin mRNR的表达显著下降(P<0.05)。结论EGCG对鼻咽癌裸鼠移植瘤可能具有放疗增敏作用,这种作用可能与凋亡抑制基因Survivin的表达下调有关。
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| 关 键 词： | 鼻咽肿瘤 肿瘤移植 EGCG 放射疗法 Survivin 小鼠 裸 |
Relationship between expression of Survivin and radio-sensitization effect of EGCG in xenotransplanted nasopharyngeal carcinoma |
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| LEI-Xun,LIU Qiang-he,KONG Zhong-yu,XIANG Qiu,GENG WAN-ping,HUANG Hui,DONG Yi-yuan,LIU Fang-xian. Relationship between expression of Survivin and radio-sensitization effect of EGCG in xenotransplanted nasopharyngeal carcinoma[J]. Journal of Preclinical Medicine College of Shandong Medical University, 2009, 0(1) |
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| Authors: | LEI-Xun LIU Qiang-he KONG Zhong-yu XIANG Qiu GENG WAN-ping HUANG Hui DONG Yi-yuan LIU Fang-xian |
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| Affiliation: | 1.Department of Otorhinolaryngology & Head and Neck Surgery;Affiliated Hospital of Guilin Medical College;2.Scientific Research & Experiment Center;Guilin Medical College;3.Department of Radioncology;Guilin 541001;Guangxi;China |
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| Abstract: | Objective To investigate the relationship between expression of Survivin and radio-sensitization effects of EGCG in the in vivo growth of nasopharyngeal carcinoma.Methods The nasopharyngeal carcinoma undifferentiated cell line(CNE-2) was subcutaneously inoculated into 20 nude mice(4-6weeks,female),and the volume and the shorter diameter of tumors were determined.When the shorter diameter of tumors reached 4-6 mm,NS,EGCG(50 mg/kg·w),radiotherapy(15 Gy),and EGCG(50 mg/kg·w) followed by radiotherapy(15 Gy) 24 ... |
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| Keywords: | NPC Neoplasm seeding EGCG Radiotherapy Survivin Mice nude |
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