| 苦参碱对K562细胞株端粒酶活性和细胞周期的影响 |
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| 作者姓名： | Zhang L Jiang J Tan R |
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| 作者单位： | 重庆医科大学临床生化教研室,香港大学生物化学系 |
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| 摘 要： | 目的为寻找新的肿瘤细胞诱导分化剂,探讨了苦参碱对Ｋ５６２细胞的诱导分化作用及其机制。方法采用ＰＣＲＥｌｉｓａ方法检测苦参碱作用前后端粒酶活性的改变,以流示细胞仪分析细胞周期的改变。结果苦参碱作用后的Ｋ５６２细胞,其端粒酶活性明显受抑,且细胞周期明显改变,Ｓ期细胞数显著降低。结论苦参碱对Ｋ５６２细胞有诱导分化作用,其机制可能与抑制端粒酶活性、阻止细胞周期的进程有关。
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| 关 键 词： | 苦参碱 K562细胞 细胞周期 细胞分化 端粒酶 |
Effects of matrine on telomerase activity and cell cycle in K562 cell |
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| Zhang L,Jiang J,Tan R.Effects of matrine on telomerase activity and cell cycle in K562 cell[J].Chinese Journal of Oncology,1998,20(5):328-329. |
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| Authors: | Zhang L Jiang J Tan R |
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| Institution: | Department of Clinical Biochemistry, Chongqing University of Medical Science. |
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| Abstract: | Objective In order to search for new tumor differentiation inducer, the effects of matrine of Chinese herbal origin on differentiation of K562 cells and its mechanism were studied. Methods Telomerase activity was determined by PCR ELISA in untreated or treated K562 cells, cell cycle phases were analyzed by fluorescence activated cell sorter analysis. Results Telomerase activity of K562 cells was significantly inhibited when treated with matrine, and cell cycle of the treated K562 cells was changed with marked decrease in S phase. Conclusion Matrine can induce differentiation of K562 cells, the mechanism of which might be related to the inhibition of telomerase activity and cell cycle arrest. |
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| Keywords: | Matrine K562 cells Cell cycle Cell differentiation Telomerase |
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