Tissue-type plasminogen activator activity in morphologically normal tissues adjacent to gastrointestinal carcinomas is associated with the degree of tumor progression |
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Authors: | Gabriel Scicolone Viviana Sanchez Liliana Vauthay Federico Fuentes Alejandro Scicolone Lorenzo Scicolone Melina Rapacioli Vladimir Flores |
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Institution: | (1) School of Medicine, Institute of Cell Biology and Neurosciences, “Prof E De Robertis”, University of Buenos Aires, Paraguay 2155, 1121 Buenos Aires, Argentina;(2) Interdisciplinary Group in Theoretical Biology, Department of Biostructural Sciences, Favaloro University, 1078 Buenos Aires, Argentina;(3) Diego Thompson Hospital, San Martin, 1650 Buenos Aires, Prov. Argentina |
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Abstract: | Purpose: To investigate whether the level of plasminogen activator (PA) activity assayed in gastrointestinal carcinomas and the “morphologically
normal tissues” adjacent to them is associated with the degree of tumor progression. Methods: Tumor and “normal tissues” were obtained from gastrointestinal surgical samples to assess urokinase-type (u-PA) and tissue-type
plasminogen activator (t-PA) activities by radial caseinolytic assay and the expression of PA inhibitor-1 (PAI-1) by ELISA.
We compared the PA system between the tumor and “normal tissues” and we investigated the existence of correlations between:
(a) PA production in the tumor and “normal tissues”, (b) different components of the PA system, and (c) PA system and the
degree of tumor progression. Results: (1) Total PA activity, u-PA activity and PAI-1 expression are significantly higher in tumor than in “normal tissues”, whereas
t-PA activity does not differ between them. (2) Total PA activity mainly correlates with u-PA activity in tumor tissues and
similarly with u-PA and t-PA activities in “normal tissues”. (3) There is a significant association between t-PA activity
in tumor and “normal tissues” and the degree of tumor progression. Conclusions: “Morphologically normal tissues” adjacent to carcinomas present abnormal t-PA activity that is associated with the degree
of tumor progression. Assaying of this activity could be useful as a predictive parameter. |
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Keywords: | Plasminogen activator Proteases activity Gastrointestinal carcinomas Tumor progression Morphologically normal tissues |
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