阿魏酸钠对血管平滑肌细胞热休克蛋白27磷酸化的影响

目的： 研究阿魏酸钠对血管紧张素II（angiotensinⅡ,AngII）与血小板源性生长因子-BB（platelet derived growth factor-BB,PDGF-BB）诱导的血管平滑肌细胞（vascular smooth muscle cells,VSMCs）中热休克蛋白27（heat shock protein 27,HSP27）磷酸化的影响。方法：采用体外培养大鼠胸主动脉平滑肌细胞；用特异性的单克隆phospho-HSP27抗体的蛋白印迹法（Western blotting）检测HSP27的活性。结果：阿魏酸钠以剂量依赖性方式抑制AngII和PDGF-BB诱导的HSP27磷酸化，在10-4 mol/L〖JP2〗时抑制作用最明显，最大抑制率分别为39.0%（P<0.05）和56.8%（P<0.01）。结论：阿魏酸钠具有明显抑制由AngII和PDGF-BB诱导的VSMCs HSP27磷酸化的作用。

Effect of sodium ferulate on heat shock protein 27 phosphorylation in vascular smooth muscle cells

AIM: To investigate the effect of sodium ferulate on phosphorylation of heat shock protein 27 (HSP27) in vascular smooth muscle cells (VSMCs) induced by angiotensin II (AngII) and platelet derived growth factor-BB (PDGF-BB). METHODS: Cultured VSMCs derived from rat thoracic aorta were used.The activity of HSP27 was evaluated by Western blotting with specific phospho-HSP27 antibody. RESULTS: The phosphorylation of HSP27 in response to AngII and PDGF-BB was suppressed by sodium ferulate in a dose-dependent manner,with maximal inhibition rates of 39.0% (P<0.05) and 56.8% (P<0.01) respectively at concentration of 10-4 mol/L. CONCLUSION: HSP27 phosphorylation induced by AngII and PDGF-BB in VSMCs may be significantly inhibited by sodium ferulate.