| 对肾综合征出血热传播媒介小盾纤恙螨的研究 |
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| 引用本文： | 钱俊英,邓小昭,张云.对肾综合征出血热传播媒介小盾纤恙螨的研究[J].中国媒介生物学及控制杂志,2009,20(6). |
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| 作者姓名： | 钱俊英 邓小昭 张云 |
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| 作者单位： | 1. 陕西省疾病预防控制中心流行病科,西安,710054
2. 南京军区军事医学研究所 |
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| 摘 要： | 目的 探索小盾纤恙螨在肾综合征出血热(HFRS)传播中的作用及传播疾病的关系.方法 在疫区捕获野鼠,采集小盾纤恙螨,用Vero-E6细胞组织培养及间接免疫荧光法(IFA)检测汉坦病毒(HV)感染情况,并用RT-PCR检测螨体内HV-RNA,观察螨体内HV增殖,原位RT-PCR分子杂交法进行螨体内HV定位.结果 IFA检测HV抗原阳性鼠寄生小盾纤恙螨HV自然感染率为66.7%,IFA检测阴性鼠寄生螨HV自然感染率为25.0%;该螨通过叮刺和经卵传播HV.结论 小盾纤恙螨为野鼠型HFRS主要传播媒介之一,在传播HFRS中的作用不容忽视.
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| 关 键 词： | 小盾纤恙螨 肾综合征出血热 传播媒介 |
Investigation of Leptotrombidium scutellare-hemorrhagic fever with renal syndrome vector |
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| QIAN Jun-ying,DENG Xiao-zhao,ZHANG Yun.Investigation of Leptotrombidium scutellare-hemorrhagic fever with renal syndrome vector[J].Chinese Journal of Vector Biology and Control,2009,20(6). |
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| Authors: | QIAN Jun-ying DENG Xiao-zhao ZHANG Yun |
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| Abstract: | Objective Explore the role of chigger mite in the transmission of hemorrhagic fever with renal syndrome (HFRS). Methods Chigger mites were collected from rata caught in the plague loci. HV was detected by Vero-E6 culture and IFA. HV-RNA from mites was teated by RT-PCR, and HV was located by hybridization histochemistry. Results The infection rate of HV was 66.7% in chigger mites collected from rats which antigens were positive, however it was only 25.0% in mites from rats which antigens were negative. The result showed that chigger mite could transmit HFRS by stinging and vertical transmission.Conclusion It indicates that chigger mite is one of HFRS vectors, which roles in HFRS transmission can not be neglected. |
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| Keywords: | Leptotrombidium scutellare Hemorrhagic fever with renal syndrome Transmission vector |
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