| 益气化瘀补肾方对大鼠气虚型颈椎病的作用机制研究 |
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| 引用本文： | 江建春,崔学军,卞琴,梁倩倩,李晨光,周泉,卢盛,周重建,施杞,王拥军.益气化瘀补肾方对大鼠气虚型颈椎病的作用机制研究[J].上海中医药大学学报,2009(4):50-53. |
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| 作者姓名： | 江建春 崔学军 卞琴 梁倩倩 李晨光 周泉 卢盛 周重建 施杞 王拥军 |
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| 作者单位： | 上海中医药大学附属龙华医院;上海中医药大学脊柱病研究所; |
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| 基金项目： | 国家杰出青年科学基金资助项目(30625043);;国家自然科学基金重大国际合作项目(30710103904);;国家自然科学基金资助项目(30572398,30600829,30701118);;上海市中药现代化重点项目(06DZ19718);;上海市卫生局中医药科研基金资助项目(2006T001A);;上海市教委创新基金资助项目(08YZ56) |
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| 摘 要： | 目的：探讨益气化瘀补肾方对气虚型颈椎病大鼠的作用机制。方法：选择3月龄雌性SPF级SD大鼠30只，随机分为正常组、气虚型颈椎病模型组（模型组）和益气化瘀补肾方组（治疗组），每组10只。正常组不施加任何手术，气虚型颈椎病模型采用动静力失衡性大鼠颈椎间盘退变模型复合疲劳加饥饱失常法气虚模型建立，手术3个月后治疗组大鼠予益气化瘀补肾方灌胃治疗1个月。通过放射免疫学、颈椎间盘组织病理学、免疫组织化学、分子生物学等技术研究益气化瘀补肾方作用机制。结果：模型组动物出现精神委靡、少动、体质量下降等气虚表现，cGMP降低，cAMP／cGMP增高，三酰甘油和CD62p增高，E2降低，颈椎间盘退变，Ⅱ型胶原减少，Agc1、Col2a1表达减少，MMP-13表达增高；治疗组与模型组相比，动物精神状态恢复稍快，cAMP／cGMP增高，三酰甘油和CD62p降低，颈椎间盘退变有所改善，Ⅱ型胶原增加，C012a1和TIMP-1表达增高。结论：益气化瘀补肾方通过免疫、代谢、凝血等系统改善证候指标并延缓椎间盘退变。
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| 关 键 词： | 益气化瘀补肾方 颈椎病 气虚证 大鼠 |
Mechanism of "Yiqi Huayu Bushen Recipe" in Treating Cervical Spondylopathy of Qi Deficiency Pattern in Rats |
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| JIANG Jian-chun CUI Xue-jun BIAN Qin LIANG Qian-qian LI Chen-guang ZHOU Quan LU Sheng ZHOU Chong-jian SHI Qi WANG Yong-junLonghua Hospital Affiliated to Shanghai University of TCM.Mechanism of "Yiqi Huayu Bushen Recipe" in Treating Cervical Spondylopathy of Qi Deficiency Pattern in Rats[J].Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai,2009(4):50-53. |
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| Authors: | JIANG Jian-chun CUI Xue-jun BIAN Qin LIANG Qian-qian LI Chen-guang ZHOU Quan LU Sheng ZHOU Chong-jian SHI Qi WANG Yong-junLonghua Hospital Affiliated to Shanghai University of TCM |
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| Institution: | JIANG Jian-chun CUI Xue-jun BIAN Qin LIANG Qian-qian LI Chen-guang ZHOU Quan LU Sheng ZHOU Chong-jian SHI Qi WANG Yong-junLonghua Hospital Affiliated to Shanghai University of TCM,Institute of Spine,Shanghai University of TCM |
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| Abstract: | Objective:To explore the mechanism of "Yiqi Huayu Bushen Recipe" (YHBR) ,a compound traditional Chinese herbal medicine,in treating cervical spondylopathy (CS) of qi deficiency pattern in rats. Methods:A total of 30 three-month-old female Sprague-Dawley rats were randomly divided into normal control,untreated group and treated group;there were ten rats in each group. The rat model of cervical spondylopathy of qi deficiency pattern was established by combining disease and pattern models. After one-month YHBR... |
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| Keywords: | "Yiqi Huayu Bushen recipe" cervical spondylopathy qi deficiency pattern rat |
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