Dried blood spots,valid screening for viral hepatitis and human immunodeficiency virus in real-life |
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Authors: | Belinda K M?ssner Benjamin Staugaard Janne Jensen S?ren Thue Lillevang Peer B Christensen Dorte Kinggaard Holm |
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Institution: | Belinda K Mössner, Benjamin Staugaard, Peer B Christensen, Department of Infectious Diseases, Odense University Hospital, 5000 Odense, Denmark;Janne Jensen, Department of Medicine, Lillebaelt Hospital, Kolding, 6000 Kolding, Denmark;Søren Thue Lillevang, Dorte Kinggaard Holm, Department of Clinical Immunology, Odense University Hospital, 5000 Odense, Denmark;Peer B Christensen, Department of Clinical Research, University of Southern Denmark, 5000 Odense, Denmark |
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Abstract: | AIMTo detect chronic hepatitis B (CHB), chronic hepatitis C (CHC) and human immunodeficiency virus (HIV) infections in dried blood spot (DBS) and compare these samples to venous blood sampling in real-life.METHODSWe included prospective patients with known viral infections from drug treatment centers, a prison and outpatient clinics and included blood donors as negative controls. Five drops of finger capillary blood were spotted on filter paper, and a venous blood sample was obtained. The samples were analyzed for HBsAg, anti-HBc, anti-HBs, anti-HCV, and anti-HIV levels as well as subjected to a combined nucleic acid test (NAT) for HBV DNA, HCV RNA and HIV RNA.RESULTSSamples from 404 subjects were screened (85 CHB, 116 CHC, 114 HIV and 99 blood donors). DBS had a sensitivity of > 96% and a specificity of > 98% for the detection of all three infections. NAT testing did not improve sensitivity, but correctly classified 95% of the anti-HCV-positive patients with chronic and past infections. Anti-HBc and anti-HBS showed low sensitivity in DBS (68% and 42%).CONCLUSIONDBS sampling, combined with an automated analysis system, is a feasible screening method to diagnose chronic viral hepatitis and HIV infections outside of the health care system. |
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Keywords: | Dried blood spot Real-life Screening Hepatitis B Hepatitis C Human immunodeficiency virus People who inject drugs Drug-users Prisoners |
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