| 洛铂对SKOV3细胞凋亡及bax、bcl-2基因表达的影响 |
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| 引用本文： | 王辉,段爱红,司军英. 洛铂对SKOV3细胞凋亡及bax、bcl-2基因表达的影响[J]. 中国热带医学, 2010, 10(1): 68-70 |
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| 作者姓名： | 王辉 段爱红 司军英 |
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| 作者单位： | 1. 北京石景山医院妇产科,北京,100043
2. 邯郸市中心医院妇产科,河北,邯郸,056000 |
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| 摘 要： | 目的研究洛泊对体外培养的浆液性卵巢癌细胞SKOV3凋亡作用及探讨其对凋亡基因bcl-2和bax表达的影响。方法体外培养卵巢癌细胞SKOV3,用MTT比色法检测洛铂对SKOV3细胞株的增殖抑制作用以及流式细胞仪检测洛铂对SKOV3细胞株的凋亡率,流式细胞仪检测凋亡相关蛋白bax、bcl-2的表达。结果体外培养卵巢癌SKOV3细胞经不同浓度洛泊处理后细胞生长明显受到抑制,洛泊作用于卵巢癌SKOV3细胞可诱导细胞的凋亡,同时,随着洛铂浓度的增加,bax基因的表达增加,而bcl-2表达减少。结论卵巢癌SKOV3细胞对洛泊具有药物敏感性,洛泊可诱导卵巢癌细胞的凋亡,bax蛋白的表达增加和bcl-2蛋白表达的减少可能是洛泊诱导卵巢癌细胞的凋亡机制之一。
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| 关 键 词： | 洛泊 卵巢肿瘤 凋亡 |
Effect of lobaplatin on apoptosis of ovarian cell SKOV3 and expression of bax and bcl-2 gene |
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| WANG Hui,DUAN Ai-hong,SI Jun-ying. Effect of lobaplatin on apoptosis of ovarian cell SKOV3 and expression of bax and bcl-2 gene[J]. China Tropical Medicine, 2010, 10(1): 68-70 |
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| Authors: | WANG Hui DUAN Ai-hong SI Jun-ying |
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| Affiliation: | 1.Department of Obstetrics and Gynecology,Shijingshan Hospital,Beijing 100043,P.R. China) |
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| Abstract: | Objective To investigate the effect of lobaplatin on apoptosis of ovarian cell and its relationship with the expression of bax and bcl-2 gene. Methods MTT assay was used to detect the effect of lobaplatin on proliferation,and apoptosis of SKOV3 cells were detectrd by flow cytometry.The expression of bax and bcl-2 protein were detected by flow cytometry and Results Lobaplatin could inhibit the proliferation of SKOV3 cells significantly. After treatment with lobaplatin,SKOV3 cells shown some typical morpholog... |
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| Keywords: | Lobaplatin Ovarian neoplasms Apoptosis |
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