Activation-induced expression of thymic shared antigen-1 on T lymphocytes and its inhibitory role for TCR-mediated IL-2 production |
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Authors: | Kosugi Atsushi; Saitoh Shin-Ichiroh; Narumiya Seiji; Miyake Kensuke; Hamaoka Toshiyuki |
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Institution: | 1 Precursory Research for Embryonic Science and Technology (PRESTO), Research Development Corporation of Japan (JRDC) 2–2, Yamada-oka, Suite, Osaka 565, Japan
2 Biomedical Research Center, Osaka University Medical School 2–2, Yamada-oka, Suite, Osaka 565, Japan
3 Department of Immunology, Saga Medical School Nabeshima, Saga 849, Japan |
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Abstract: | We have produced a hamster mAb, PRST1, which reacts with thymicshared Ag-1 (TSA-1), a product of the Ly6 gene family. By cross-blockingexperiments, we found that TSA-1 is identical to stem cell Ag-2(Sca-2). Using PRST1, the changes of TSA-1/Sca-2 expressionon mature T cells during the activation process were analyzed.Although freshly isolated T cells did not express detectableTSA-1 on their cell surface, in vitro stimulation of T cellswith concanavalln A induced a marked increase of surface TSA-1expression. The increased expression of TSA-1 on T cells wasdetected from 12 h after stimulation and was associated withthe increase of TSA-1 mRNA. In vivo injection of mice with staphylococcalenterotoxin B (SEB) resulted in the enhanced TSA-1 expressionin splenic Vß8+ T cells. This antigen-specific inductionof TSA-1 expression in vivo preceded a detectable increase innumbers of Vß8+ T cells after SEB injection. Functionally,whereas anti-TSA-1 mAb was not mitogenic to T cells, it inhibitedanti-CD3-induced IL-2 production by T cell hybridomas. Theseresults indicate that TSA-1/Sca-2 is a unique marker for T cellactivation and a signal through this molecule may have a negativefeedback role to limit IL-2 production from activated T cellsstimulated through the TCR. |
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Keywords: | glycosylphosphatidylinositol-anchored protein Ly6 gene family stem cell Ag-2 T cell activation |
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