| Short-term effects of di-（2-ethylhexyl） phthalate on testes,liver, kidneys and pancreas in mice |
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| 作者姓名： | Miura Y Naito M Ablake M Terayama H Yi SQ Qu N Cheng LX Suna S Jitsunari F Itoh M |
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| 作者单位： | [1]Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, Japan [2]Department of Histology and Embryology, Xin Jiang Medical University, Xinjiang 830001, China [3]Department of Hygiene and Public Health, Faculty of Medicine, Kagawa University, Kagawa 761-0783, Japan |
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| 基金项目： | Acknowledgment The authors wish to thank Mrs Miyuki Kitaoka, Mrs Yuki 0gawa and Mrs Fumiko Komoda for their excellent secretarial and technical supports. This work was supported by a Grant-in-Aid for General Scientific Research （17591704） from the Ministry of Education, Science, Sports and Culture, Japan. |
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| 摘 要： | Aim： To determine the biochemical effect of di-（2-ethylhexyl） phthalate （DEHP） on testes, liver, kidneys and pancreas on day 10 in the process of degeneration of the seminiferous epithelium. Methods： Diets containing 2% DEHP were given to male Crlj：CDI（ICR） mice for 10 days. The dose of DEHP was 0.90±0.52 mg/mouse/day. Their testes, livers, kidneys and pancreata were examined for detection of mono-（2-ethylhexyl） phthalate （MEHP）, nitrogen oxides （NOx） produced by peroxidation of nitric oxide （NO） with free radicals, and lipid peroxidation induced by the chain reaction of free radicals. Results： Histological observation and serum analysis showed the presence of severe sperrnatogenic disturbance, Leydig cell dysfunction, liver dysfunction and dehydration. Unexpectedly, the concentration of MEHP in the testes was extremely low compared with that in the liver. However, the concentration of the NOx in the testes was as high as the hepatic concentration. Furthermore, free radical-induced lipid peroxidation was histochemically detected in the testes but not in the liver. Conclusion： The results indicate that DEHP-induced aspermatogenesis is caused by the high sensitivity of the testicular tissues to MEHP rather than the specific accumulation or uptake of circulating MEHP into the testes.
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| 关 键 词： | 邻苯二甲酸酯 睾丸 肝脏 肾脏 胰腺 短期影响 生化作用 小白鼠 |
| 修稿时间： | 2006-01-242006-06-05 |
Short-term effects of di-(2-ethylhexyl) phthalate on testes, liver, kidneys and pancreas in mice |
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| Miura Y,Naito M,Ablake M,Terayama H,Yi SQ,Qu N,Cheng LX,Suna S,Jitsunari F,Itoh M.Short-term effects of di-(2-ethylhexyl) phthalate on testes, liver, kidneys and pancreas in mice[J].Asian Journal of Andrology,2007,9(2):199-205. |
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| Authors: | Miura Yumi Naito Munekazu Ablake Maira Terayama Hayato Yi Shuang-Qin Qu Ning Cheng Lin-Xian Suna Shigeru Jitsunari Fumihiko Itoh Masahiro |
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| Institution: | Department of Anatomy, Tokyo Medical University, Shinjuku-ku, Tokyo 160-8402, Japan. |
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| Abstract: | AIM: To determine the biochemical effect of di-(2-ethylhexyl) phthalate (DEHP) on testes, liver, kidneys and pancreas on day 10 in the process of degeneration of the seminiferous epithelium. METHODS: Diets containing 2% DEHP were given to male Crlj:CD1(ICR) mice for 10 days. The dose of DEHP was 0.90 +/- 0.52 mg/mouse/day. Their testes, livers, kidneys and pancreata were examined for detection of mono-(2-ethylhexyl) phthalate (MEHP), nitrogen oxides (NOx) produced by peroxidation of nitric oxide (NO) with free radicals, and lipid peroxidation induced by the chain reaction of free radicals. RESULTS: Histological observation and serum analysis showed the presence of severe spermatogenic disturbance, Leydig cell dysfunction, liver dysfunction and dehydration. Unexpectedly, the concentration of MEHP in the testes was extremely low compared with that in the liver. However, the concentration of the NOx in the testes was as high as the hepatic concentration. Furthermore, free radical-induced lipid peroxidation was histochemically detected in the testes but not in the liver. CONCLUSION: The results indicate that DEHP-induced aspermatogenesis is caused by the high sensitivity of the testicular tissues to MEHP rather than the specific accumulation or uptake of circulating MEHP into the testes. |
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| Keywords: | phthalatc nitrogen oxide free radical testis |
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