| Age-dependent expression of the cystatin-related epididymal spermatogenic （Cres） gene in mouse testis and epididymis |
| |
| 作者姓名： | Yuan Q Guo QS Cornwall GA Xu C Wang YF |
| |
| 作者单位： | [1]Department of Histology ＆ Embryology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China [2]Departments of Cell Biology and Biochemistry; Texas Technology University Health Science Center, Lubbock, TX, USA |
| |
| 基金项目： | This work was supported by the National Natural Sciences Foundation of China （No. 30070391）, the Science and Technology Development Foundation of Shanghai Population and Family Planning Commission （No. 03JG 05009）, and the Science and Technology Foundation of Shanghai Jiao Tong University. |
| |
| 摘 要： | Aim： To investigate the spatial and temporal expression of the cystatin-related epididymal spermatogenic （Cres） gene in mouse testis and epididymis during postnatal development. Methods： The QuantiGene assay and indirect immunofluorescence technique were used to examine the Cres mRNA and Cres protein level in mouse testis and epididymis on postnatal days 14, 20, 22, 28, 35, 49, 70 and 420. Results： （1） In both the testis and epididymis, Cres mRNA was fast detected on day 20, then it increased gradually from day 20 to day 70, and the high expression level maintained till day 420. （2） In the testis, the Cres protein was exclusively localized to the elongating spermatids and was first detected on day 22. The number of Cres-positive spermatids increased progressively till day 49. From day 49 to day 420, the number of Cres-positive cells was almost stable. （3） The Cres protein was first detected on day 20 in the proximal caput epididymal epithelium. By day 35, the expression level of the Cres protein increased dramatically and the high level was maintained till day 420. Moreover, the luminal fluid of the midcaput epididymis was also stained Cres-positive from day 35 on. No Cres-positive staining was observed in distal caput, corpus and cauda epididymis throughout. Conclusion： The Cres gene displays a specific age-dependent expression pattern in mouse testis and epididymis on both the mRNA and protein level.
|
| 关 键 词： | 鼠睾丸 附睾精子发生基因 胱蛋白酶抑制剂 基因表达 年龄 |
| 修稿时间： | 2006-07-212006-12-12 |
Age-dependent expression of the cystatin-related epididymal spermatogenic (Cres) gene in mouse testis and epididymis |
| |
| Yuan Q,Guo QS,Cornwall GA,Xu C,Wang YF.Age-dependent expression of the cystatin-related epididymal spermatogenic (Cres) gene in mouse testis and epididymis[J].Asian Journal of Andrology,2007,9(3):305-311. |
| |
| Authors: | Yuan Qing Guo Qiang-Su Cornwall Gail A Xu Chen Wang Yi-Fei |
| |
| Institution: | Department of Histology and Embryology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China. |
| |
| Abstract: | AIM: To investigate the spatial and temporal expression of the cystatin-related epididymal spermatogenic (Cres) gene in mouse testis and epididymis during postnatal development. METHODS: The QuantiGene assay and indirect immunofluorescence technique were used to examine the Cres mRNA and Cres protein level in mouse testis and epididymis on postnatal days 14, 20, 22, 28, 35, 49, 70 and 420. RESULTS: (1) In both the testis and epididymis, Cres mRNA was first detected on day 20, then it increased gradually from day 20 to day 70, and the high expression level maintained till day 420. (2) In the testis, the Cres protein was exclusively localized to the elongating spermatids and was first detected on day 22. The number of Cres-positive spermatids increased progressively till day 49. From day 49 to day 420, the number of Cres-positive cells was almost stable. (3) The Cres protein was first detected on day 20 in the proximal caput epididymal epithelium. By day 35, the expression level of the Cres protein increased dramatically and the high level was maintained till day 420. Moreover, the luminal fluid of the midcaput epididymis was also stained Cres-positive from day 35 on. No Cres-positive staining was observed in distal caput, corpus and cauda epididymis throughout. CONCLUSION: The Cres gene displays a specific age-dependent expression pattern in mouse testis and epididymis on both the mRNA and protein level. |
| |
| Keywords: | cystatin-related epididymal spermatogenic gene spermatogenesis sperm maturation development |
| 本文献已被 维普 PubMed 等数据库收录！ |
|
