胃癌患者血清miR-300的表达及临床意义

目的探讨胃癌患者血清中miR-300的表达水平及miR-300作为胃癌诊断标记物的可能性。方法采用实时定量RT-PCR方法检测25例胃癌患者及15例健康志愿者血清中miR-300的表达水平;采用全自动化学发光免疫分析法,检测上述标本的血清癌胚抗原（CEA）、癌抗原125（CA125）、癌抗原199（CA199）及癌抗原153（CA153）水平。结果 25例胃癌患者miR-300表达水平是正常人的23.4倍,两者差异有统计学意义（P=0.024）;胃癌患者血清CEA、CA125、CA199及CA153水平显著高于对照组（均P〈0.05）;在胃癌的诊断中miR-300的敏感性比CEA、CA125、CA199及CA153高,但特异性较低。结论血清miR-300在胃癌中的表达水平高于正常人,对于胃癌的诊断具有一定价值。

The expression and clinical significance of miceoRNA-300 in serum of patients with gastric cancer

Objective Toinvestigate theexpression levelofmiR-300 in serumofpatientswith gastriccancerandto explore the value of miR-300 in the diagnosis of gastric cancer. Methods Real-time quantitative RT-PCR（qRTPCR） was performed to detect miR-300 level of 25 gastric cancer patients and 15 healthy volunteers. Automatic chemiluminescence immunoassay was used to detect the serum level of CEA, CA125, CA199 and CA153. Results The expression of miR-300 in 25 gastric cancer patients was 23.4 times as more as the normal controls. Compared with the control group, the levels of CEA, CA125, CA199 and CA153 were higher in patients with gastric cancer. In the diagnosis of gastric cancer, the sensitivity of miR-300 was higher than that of CEA, CA125, CA199 and CA153,but the specificity of miR-300 was lower than that of CEA, CA125, CA199 and CA153. Conclusions Expression level of serum miR-300 in gastric cancer patients was significantly higer than that in normal controls, and it has diagnosis value in gastric cancer.