| Ⅱ型单纯疱疹病毒糖蛋白D在杆状病毒表达系统中的表达、纯化及免疫效果评价 |
| |
| 引用本文： | 姜德玉,刘坤,张伟,周朋,李君丽,王希良,于三科.Ⅱ型单纯疱疹病毒糖蛋白D在杆状病毒表达系统中的表达、纯化及免疫效果评价[J].免疫学杂志,2010(6). |
| |
| 作者姓名： | 姜德玉 刘坤 张伟 周朋 李君丽 王希良 于三科 |
| |
| 作者单位： | 西北农林科技大学动物医学学院;军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室; |
| |
| 摘 要： | 目的:利用Bac-to-Bac杆状病毒表达系统对Ⅱ型单纯疱疹病毒(HSV-2)糖蛋白D(gD2)进行表达,纯化后评价其免疫效果,以探索HSV-2重组亚单位疫苗的研制。方法:提取HSV-2DNA作为模板,用聚合酶链式反应(PCR)扩增gD2基因,克隆至pFastBac HTA载体中,利用载体中的His基因标记gD2,通过Bac-to-Bac杆状病毒表达系统表达His-gD2融合蛋白,表达产物进行SDS-PAGE和Western-blot检验,用镍柱进行纯化,免疫小鼠评价其免疫原性。结果:HSV-2gD2在昆虫细胞Sf9中得到特异性表达,纯化后的重组蛋白诱导小鼠体内产生高水平的IgG抗体及中和抗体。结论:gD2基因在Bac-to-Bac杆状病毒表达系统中获得表达,表达产物表现出良好的免疫原性及中和活性,为发展HSV-2亚单位疫苗奠定了基础。
|
| 关 键 词： | Ⅱ型单纯疱疹病毒 糖蛋白D Bac-to-Bac杆状病毒表达系统 免疫原性 中和活性 |
Expression of the herpes simplex virus type 2 glycoprotein D in baculovirus expression system and evaluation of its immunogenicity |
| |
| JIANG Deyu,LIU Kun,ZHANG Wei,ZHOU Peng,WANG Xiliang,YU Sanke College of Veterinary Medicine,Northwest A&F University,Yangling ,China.Expression of the herpes simplex virus type 2 glycoprotein D in baculovirus expression system and evaluation of its immunogenicity[J].Immunological Journal,2010(6). |
| |
| Authors: | JIANG Deyu LIU Kun ZHANG Wei ZHOU Peng WANG Xiliang YU Sanke College of Veterinary Medicine Northwest A&F University Yangling China |
| |
| Institution: | JIANG Deyu,LIU Kun,ZHANG Wei,ZHOU Peng,WANG Xiliang,YU Sanke College of Veterinary Medicine,Northwest A&F University,Yangling 712100,China |
| |
| Abstract: | To explore the possibility of preparing a subunit vaccine to prevent the infection of HSV-2, we expressed HSV-2 glycoprotein D (gD2) by Bac-to-Bac baculovirus expression system and evaluated its immunogenicity by immunizing Balb/c mice. HSV-2 DNA was extracted and used as template in polymerase chain reactions (PCR) to amplify the gD2 gene. Then the PCR product was cloned into the pFastBac HTA vector, and the Bac-to-Bac expression system was used to express HSV-2 gD with his-tag in insect cells. The express... |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
|
