内皮素1在肺癌组织中的表达

目的：研究内皮素1（ET-1）在肺癌组织中的表达与肺癌分型及分级的关系。方法：采用免疫组织化学ABC染色及图像分析技术测定ET—1阳性率及含量。结果：104例肺癌组织ET—1阳性表达主要分布在胞浆内。腺癌、鳞癌和大细胞肺癌ET—1表达较高，表达阳性率分别为71．4％（20／28）、57．1％（16／68）及40．0％（8／20）。小细胞肺癌表达最低，表达阳性率为21．4％（6／28）。腺癌、鳞癌阳性表达的图像分析表明，肿瘤分化程度越低ET—1含量越高，肿瘤分化程度越高ET—1含量越低。结论：ET—1普遍存在于肺癌细胞中。腺癌和鳞癌高表达，ET—1含量可能与肿瘤分化程度有一定关系。ET—1有望作为肺腺癌、鳞癌的一个检测指标。

Expression of endothelin-1 in lung cancer tissues

Objective: To study the endothelin-1(ET-1) expression in lung cancer and its correlation with the tumor type and grade.Methods: ET-1 expression was detected by avidin-biotin-peroxidase complex method and image analysis technology.Results: The positive expression of ET-1 was mainly located in the cytoplasm in 104 lung cancer tissues.The positive rates of ET-1 in adenocarcinoma,squamous-cell carcinoma and large-cell lung cancer were 74.1%,57.1% and 40.0%,respectively.The positive rate of ET-1 in small-cell lung cancer was 21.4%,significantly lower than that in nonsmall-cell lung cancer.The result of image analysis on adenocarcinomaand squamous-cell carcinoma showed that the lower tumor differentiation,the higher ET-1 expression level.Conclusion:The ET-1 expression level may be related to the differentiation degree of lung cancer.