Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus |
| |
Authors: | Kaur Amitinder Sanford Hannah B Garry Deirdre Lang Sabine Klumpp Sherry A Watanabe Daisuke Bronson Roderick T Lifson Jeffrey D Rosati Margherita Pavlakis George N Felber Barbara K Knipe David M Desrosiers Ronald C |
| |
Institution: | New England Primate Research Center, Harvard Medical School, One Pine Hill Drive, P.O. Box 9102, Southborough, MA 01772-9102, USA. amitinder_kaur@hms.harvard.edu |
| |
Abstract: | The immunogenicity and protective capacity of replication-defective herpes simplex virus (HSV) vector-based vaccines were examined in rhesus macaques. Three macaques were inoculated with recombinant HSV vectors expressing Gag, Env, and a Tat-Rev-Nef fusion protein of simian immunodeficiency virus (SIV). Three other macaques were primed with recombinant DNA vectors expressing Gag, Env, and a Pol-Tat-Nef-Vif fusion protein prior to boosting with the HSV vectors. Robust anti-Gag and anti-Env cellular responses were detected in all six macaques. Following intravenous challenge with wild-type, cloned SIV239, peak and 12-week plasma viremia levels were significantly lower in vaccinated compared to control macaques. Plasma SIV RNA in vaccinated macaques was inversely correlated with anti-Rev ELISPOT responses on the day of challenge (P value<0.05), anti-Tat ELISPOT responses at 2 weeks post challenge (P value <0.05) and peak neutralizing antibody titers pre-challenge (P value 0.06). These findings support continued study of recombinant herpesviruses as a vaccine approach for AIDS. |
| |
Keywords: | AIDS vaccine Recombinant HSV vectors DNA prime SIV Rhesus macaque |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|