离子对高效液相色谱法测定尿囊素乳膏中尿囊素含量

目的:改进尿囊素乳膏中尿囊素含量的测定方法,为产品质量控制提供依据.方法:采用离子对高效液相色谱法测定,色谱柱为ODS-C18柱(250mm×4.6 mm,5 μm),流动相为0.005 mol·L-1 磷酸二氢钾溶液-甲醇(100:1)(内含0.038%的庚烷磺酸钠);流速:1.0 ml·min-1;柱温:30℃;检测波长为210 nm.结果:尿囊素在10.08~40.32 μg·ml-1范围内有良好的线性关系(r=0.9999),平均回收率为99.6%(RSD=1.12%,n=9).结论:该方法准确易行,便于质量控制.

Determination of Allantoin in Allantoin Creams by Ion pair HPLC

Objective:To improve a method for the determination of allantoin in allantoin creams for the quality control.Method: The determination was performed on an ODS-C₁₈column（250 mm×4.6 mm,5μm）with the mobile phase of 0.005 mol · L^-1sodium dihydrogen phosphate-methanol（100:1）（containing 0.038%sodium 1-heptanesulfonate）.The flow rate was 1.0 ml · min^-1,the column temperature was 30℃and the detection wavelength was 210 nm.Result:The linear curve of allantoin was obtained within the range of 10.0840.32 g · ml^-1（r=0.999 9）,and the average recovery was 99.56%（RSD=1.12%,n=9）.Conclusion:The improved method is accurate and feasible.It can be used conveniently in the quality control of the preparation.