Mutations in the structural gene for cytochrome c result in deficiency of both cytochromes aa
3
and c in Neurospora crassa |
| |
Authors: | Drell A Bottorff Sukran Parmaksizoglu Edmond G Lemire John W Coffin Helmut Bertrand Frank E Nargang |
| |
Institution: | (1) Department of Genetics, University of Alberta, T6G 2E9 Edmonton, Alberta, Canada;(2) Department of Microbiology, Michigan State University, 48824-1011 East Lansing, MI, USA |
| |
Abstract: | The cyt-12-12 mutant of Neurospora crassa is characterized by slow growth and a deficiency of spectrophotometrically-detectable cytochromes aa
3
and c. Using a sib-selection procedure we have isolated the cyt-12
+
allele from a cosmid library of N. crassa genomic DNA. Characterization of the cyt-12
+
allele reveals that it encodes the structural gene for cytochrome c. DNA sequence analysis of the cyt-12-12 allele revealed a mutation in the cytochrome c coding sequence that results in replacement of a glycine residue, which is invariant in the cytochrome c of other species, with an aspartic acid. Genetic analysis confirms that cyt-12-12 is allelic with the previously-characterized cyc-1-1 mutant, which was also shown to affect the single locus encoding cytochrome c in N. crassa. We suggest that the amount of functional cytochrome c present in mitochondria influences the level of cytochrome aa
3
. |
| |
Keywords: | Cytochrome c Cytochrome aa
3
Mitochondria Neurospora crassa |
本文献已被 SpringerLink 等数据库收录! |
|