高脂饮食诱导肥胖大鼠瘦素、增食欲素及有关激素相互关系

目的:研究高脂饮食诱导肥胖大鼠瘦素、增食欲素及有关激素之间的相互关系。方法:选取断乳约1周、体重85～95g的Wistar雄性大鼠60只,分为对照组、轻、中、重度肥胖组各15只,检测血清瘦素、增食欲素、胰岛素、生长激素和血脂水平。结果:瘦素随着体重的增加其含量减少;增食欲素和胰岛素水平随着体重的增加而增多;生长激素改变无规律性。体重、Lee指数、增食欲素均与TC、TG、LDL鄄C及胰岛素呈显著正相关穴P<0.01雪,与瘦素均呈负相关穴P<0.01雪,与生长激素无相关性。结论:肥胖大鼠瘦素水平降低,增食欲素及胰岛素水平增加,提示用重组瘦素治疗肥胖症具有潜在的应用前景。

Relations among Leptin,Orexin and related hormones in differential obese rats induced by high-fat diet

Objective: To study the relations among Leptin, Orexin and related hormones of differential obese rats induced by high-fat diet. Methods: Sixty male rats whose weight was between 85g and 95g were weaned for a week and divided into four groups, control group, mild, moderate and severe obese groups. There were 15 rats in each group and their blood levels of Leptin, OrexinA, insulin, growth hormone and TC, TG, HDL-C were measured. Results: The Leptin level decreased but the OrexinA and insulin level of serum increased with the increase of weight. The GH of serum didn't show regulative change with the change of weight. The Orexin, insulin and TC, TG, LDL-C of serum positively correlated with body weight and Lee index, but they negatively correlated with Leptin level. The Orexin and insulin levels of serum positively correlated with the levels of TC, TG, LDL-C. The levels of Leptin, OrexinA, insulin and TC, TG, LDL-C had no association with the level of growth hormone(GH) level. Conclusion: High-fat diet may contribute to obesity by reducing Leptin and improving Orexin and insulin, so recombinant Leptin may have a potential prospect in treatment of obsity.