明党参化学成分分布规律研究

目的:研究明党参植株内化学成分的分布规律,为栽培调控提供理论依据。方法:运用HPLC和比色法对明党参植株内不同部位的多糖、甘露醇、胆碱、生理性灰分和水溶性成分进行分析,并用夹角余弦法计算水溶性成分的相似系数。结果:茎叶与根中胆碱、多糖、水溶性浸出物和生理性灰分含量均差异显著,根部胆碱与多糖的含量高于茎叶,茎叶中水溶性浸出物和生理性灰分的含量高于根部。根的不同部位间甘露醇、多糖、水溶性浸出物和生理性灰分含量均差异显著,各成分的含量在根的纵向上呈自上而下递减的趋势;横向上甘露醇、多糖和水溶性浸出物的含量呈自外向内递增的趋势,生理性灰分含量与之相反,向内递减;横向部位间差异大于纵向部位。茎叶中水溶性成分总峰面积是根的2.81倍;中柱中水溶性成分总峰面积显著高于皮层和表皮。结论:明党参茎叶中化学成分总量显著高于根部;根部药用品质自内向外降低,自上向下降低。

Distribution of chemical constituents in Changium smyrnioides

Objective : To analyze the distribution of chemical constituents in different parts of Changium smyrnioides. Method : The content of polysaccharides,mannitol and choline was determined by colorimetry and the water-soluble components was determined by HPLC. similarity coefficient of water-soluble components was calculated by the included angle cosine method. Result : The Contents of chemical constituents were significantly different between the leaves and the roots.The contents of choline and polysaccharides in the roots was higher than those in the leaves,but the contents of water-soluble extract and physiological ash in the leaves was higher than those in the roots.Contents of chemical constituents were also significantly different at different segments in the root,which decreased from the upper to the lower parts,increased from the outside to the inside parts except physiological ash,and the difference in horizontal segments was higher than that in vertical segments.Total peak area of water-soluble components in the leaves was 2.81 times higher than that in the roots,and gradually increased from the outside to the inside parts. Conclusion : Total content of chemical constituents in the leaves was significantly higher than that in the roots.The quality of medicinal used parts decreased gradually from the inside to the outside parts and from the upper to the lower parts in the root of Ch. smyrnioides.