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| 人参中8种皂苷的HPLC测定 | |
| 引用本文： | 曹俊岭,李祖伦,付强,鄢丹,廖庆文,肖小河. 人参中8种皂苷的HPLC测定[J]. 中药材, 2006, 29(10): 1038-1040 |
| 作者姓名： | 曹俊岭 李祖伦 付强 鄢丹 廖庆文 肖小河 |
| 作者单位： | 1. 解放军第302医院中医药研究所,北京,100039;成都中医药大学药学院,四川,成都,610075 2. 成都中医药大学药学院,四川,成都,610075 3. 湖南中医学院药学院,湖南,长沙,410004 4. 解放军第302医院中医药研究所,北京,100039 |
| 基金项目： | 国家自然科学基金资助项目(30500670) |
| 摘 要： | 目的:为8种人参皂苷的含量测定建立一套方法。方法:色谱柱:ODS柱(Krom asil 250mm×4.6mm,5μm),DAD检测器;流动相:A为乙腈,B为水,梯度洗脱0~35 m in 19%A,35~55 m in 19%~29%A,55~75 m in29%A,75~100 m in 29%~40%A。流速:1 m l/m in,温度:室温,检测波长:203 nm。结果:用本方法对各种皂苷的测定结果:稳定性试验RSD为0.55%~2.26%,精密度试验RSD为0.85%~1.93%,重复性试验RSD为0.97%~2.72%。结论:本法稳定性好,精确度高,重复性好,可作为测定这8种皂苷的一个较好的方法。 |
| 关 键 词： | HPLC 人参皂苷 含量测定 |
| 文章编号： | 1001-4454(2006)10-1038-03 |
| 收稿时间： | 2006-03-23 |
| 修稿时间： | 2006-03-23 |
The Determination of Contents of 8 Ginsenosides in Extraction of Panax ginseng by HPLC |
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| CAO Jun-ling,LI Zu-lun,Fu Qiang,YAN Dan,LIAO Qing-wen,XIAO Xiao-he. The Determination of Contents of 8 Ginsenosides in Extraction of Panax ginseng by HPLC[J]. Journal of Chinese medicinal materials, 2006, 29(10): 1038-1040 | |
| Authors: | CAO Jun-ling LI Zu-lun Fu Qiang YAN Dan LIAO Qing-wen XIAO Xiao-he |
| Affiliation: | Institute of Chinese Medicine, 302 Hospital of PLA, Beijing 100039, China. cj10506@sina.com |
| Abstract: | OBJECTIVE: To establish a method to determine the contents of 8 ginsenosides in extraction of Panax ginseng by HPLC. METHODS: The sample was analyzed on an ODS chromatogram column (Kromasil 250 mm x 4.6 mm, 5 microm), with mobile phase of acetonitrile-water (gradient elution) at flow rate 1.0 ml/min and detection at wavelength of 203 nm. RESULTS: RSD of stability, precision and recurrency was 0.55%-2.26%, 0.85%-1.93% and 0.97%-2.72% respectively. CONCLUSION: This method can be good for the content determination of ginsenoside. |
| Keywords: | HPLC Ginsenoside Content determination |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |