人宫颈癌及慢性宫颈炎组织内P_(53)基因变异的研究

本文应用多聚酶链反应—单链构象多态性(PCR-SSCP)分析,对人宫颈癌(cervical carcinoma)和慢性宫颈炎(chronic cervicitis)组织内的P_(53)基因5-6、7-8、9外显子(exon)变异进行研究。结果表明:35例宫颈癌组织中8例出现了异常电泳带,总变异率22.86％(8/35)。其中,5-6外显子2例,变异率为5.71％(2/35);7-8外显子5例,变异率为14.29％(5/35),是变异率最高的基因片段;9外显子1例,变异率为2.86％(1/35)。8例变异标本中3例P_(53)5-6、7-8外显子同时发生突变,占变异标本的37.5％(3/8),占宫颈癌组织总标本的8.57％(3/35)。17例慢性宫颈炎组织P_(53)5-6、7-8、9外显子均未出现异常。提示:PCR-SSCP技术能有效地检出宫颈组织P_(53)基因5-9外显子变异的情况;P_(53)5-9变异是人宫颈癌发生发展过程中的一个重要事件和生物学行为;宫颈癌组织P_(53)变异多发生在5-8外显子,有不同外显子同时变异的现象;慢性宫颈炎组织无P_(53)5-6、7-8、9外显子变异现象,说明该病的发生发展与抑癌基因P_(53)外显子变异无关。

Research of mutation in P_53 gene in human cervical carcinoma and chronic cervicitis tissues

By usign PCR - SSCP technology this paper detected the mutations of exon 5-6,7-8,9 in P53 gene in cervical carcinoma and chronic cervicitis tissues. The mutation was identified in 8 out of 35 cases, the total mutation rate was 22.86% (8/35). 2 cases were found in exon 5-6,5 in exon 7-8,1 in exon 9, the mutation rates were 5.71 % (2/ 35), 14. 29 (5/35) and 2. 86 (1/35) respectively. In addition, 3 cases were shown co - mutation in exon 5-6 and 7-8 at the same time.The co - mutation rate was 37.5% in mutation samples (8 cases) and 8.57% in 35 cases of cervical carcinoma tissues. The exon 5-9 in P53 gene were not detected mutation in chronic cervicitis. PCR - SSCP method is effective to detect gene mutation. P53 gene mutation was an important affair in the progression and development of cervical carcinoma. Mutation used to occur in exon 5-8, Further more, there were different exon co-mulatoin, occurred.