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A PCR Specific for Escherichia coli O157 Based on the rfb Locus Encoding O157 Lipopolysaccharide
Authors:Patricia M. Desmarchelier   Sima S. Bilge   Narelle Fegan   Leanne Mills   James C. Vary   Jr.     Phillip I. Tarr
Affiliation:Commonwealth Scientific and Industrial Research Organisation, Brisbane, Australia,1. and Children’s Hospital and Medical Center and the University of Washington, Seattle, Washington2.
Abstract:A PCR was developed for the detection of Escherichia coli O157 based on the rfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected <1 CFU of E. coli O157 per ml in raw milk following enrichment.
Keywords:
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