人类肺癌转移相关基因的筛选

目的:应用基因芯片研究人高、低转移肺巨细胞癌细胞株95-D和95-C的基因表达谱的差异,并筛选肺癌转移相关基因。方法:分别提取并纯化两细胞株的mRNA,运用基因芯片技术得到95-D和95-C细胞的差异表达基因谱。用反转录聚合酶链反应(RT-PCR)方法对部分有差异表达的基因进行分析验证。结果:在95-D和95-C细胞中有表达差异2倍以上的基因共389条,其中表达上调的121条,表达下调的268条。RT-PCR的验证结果与芯片的筛查结果基本相符。结论:人高、低转移肺巨细胞癌细胞株95-D和95-C在基因表达谱上存在显著差异,这种差异表达的意义有待进一步研究。

Screening of human lung cancer metastasis-associated genes

Objective:To analyze differences in the gene expression profiling between human lung giant cell carcinoma cell strains of high metastatic 95-D and low metastatic 95-C,and to screen lung cancer metastasis-associated genes using cDNA microarray.Methods:The mRNAs were extracted from 95-D cell and 95-C cell respectively.We used genechip to obtain profiling of genes differentially expressed in the two cell lines.The expression changes of a part of genes determined in genechip were further identified by RT-PCR.Results:The analysis of genechip showed that the expression of 389 genes were changed.Among them,121 genes were up-regulated and 268 genes were down-regulated.The results of RT-PCR were coincided well with that of gene chip.Conclusions: there are significant differences in gene expression profiling between two cell lines,and the meaning of difference in expression profiling is under investigation.