槐杞黄对IgA肾病大鼠蛋白尿及肾组织nephrin及podocin的影响

目的观察槐杞黄对IgA肾病（IgAnephropathy，IgAN）大鼠蛋白尿及肾组织nephrin及podocin的影响，探讨槐杞黄对IgA肾病大鼠蛋白尿的治疗作用及其机制。方法将24只雄性Sprague—Dawley（SD）大鼠按随机数字表法分为对照组、模型组、槐杞黄组，每组8只。模型组和槐杞黄组建立1gA肾病模型，槐杞黄组给予槐杞黄颗粒治疗。应用定量尿沉渣计数法检测尿红细胞个数，考马斯亮蓝法检测24h尿蛋白定量，全自动生化分析仪检测血生化指标。应用直接免疫荧光法检测肾小球IgA沉积强度，间接免疫荧光法检测肾小球nephrin及podocin蛋白的表达及分布，采用实时荧光定量PCR技术检测肾皮质nephrinmRNA和podocinmRNA的表达。结果①槐杞黄组大鼠的尿红细胞计数、尿蛋白，较模型组显著减少，差异有显著意义（P〈0．05）；②槐杞黄组大鼠肾组织病变，较模型组减轻；③槐杞黄组大鼠肾小球nephrin表达量，较模型组显著升高，差异有显著意义（P〈0．01），podocin表达量较模型组稍有下降，但差异无显著意义（P〉0．05）；槐杞黄组大鼠肾皮质nephrinmRNA及podocinmRNA的表达，较模型组显著升高，差异有显著意义（P〈0．01）；④槐杞黄组大鼠肾小球nephrin及podocin的不连续斑片状、团块状异常分布，较模型组改善。结论IgA肾病大鼠肾组织nephrin及podocin出现表达变化及分布异常，槐杞黄能降低IgAN大鼠尿蛋白，其作用可能与调节nephrin及podocin的表达及分布有关。

Effects of Huaiqihuang on Proteinuria and Nephrin, Podocin in Rats With IgA Nephropathy

Objective To observe effects of huaiqihuang on proteinuria in rats with IgA nephropathy （IgAN） and dynamic changes of expression and distribution of nephrin, podocin, and to further explore the mechanism of proteinuria of IgA nephropathy and try to find the approach to the therapy for IgA nephropathy. Methods A total of 24 Sprague-Dawley （SD） rats with 6 weeks （=24） were randomly divided into 3 groups： Control group （n：8）, model group （n=8） and huaiqihuang group （n=8）. IgA nephropathy model was induced by the improved method of bovine serum albumin （BSA）＋ carbon tetraehloride （CCl4）＋lipopolysaccharide （LPS）. At the end of the experiment, hematuria, proteinuria and renal pathology should be noticed. The IgA intensity was examined by direct immunofluorescence. The protein expression and distribution of nephrin and podocin were examined by indirect immunofluorescence. The mRNA expression of nephrin and podocin were examined by real-time PCR. Results （1） The count of urinary red cells and urinary protein in huaiqihuang group were significant less than those of model group （P〈0.05）. （2）Mesangial proliferation, foot processes fusion and IgA deposition in glomeruli of huaiqihuang group were less serious than those of model group. （3）The protein expressions of nephrin in huaiqihuang group were significant higher than those in model group （P〈 0. 01）. Podocin protein expression in huaiqihuang group had no significant difference from those of model group （P〉0.05）, but were still higher than those of control group. In huaiqihuang group, and mRNA of nephrin and podocin expressed markedly higher than those of model group （P〈0.01）. （4） Abnormal discontinuous distribution of nephrin and podoein in huaiqihuang group was slighter than that in model group. Conclusion Huaiqihuang is good for decreasing urinary protein by regulating the expression and distribution of nephrin and podoein.