| 三氧化二砷对NB4细胞珠端粒酶活性的影响 |
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| 引用本文： | 郝云良,徐从高. 三氧化二砷对NB4细胞珠端粒酶活性的影响[J]. 山东大学学报(医学版), 2002, 40(6): 565-567 |
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| 作者姓名： | 郝云良 徐从高 |
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| 作者单位： | 1. 济宁市第一人民医院
2. 山东大学齐鲁医院] |
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| 摘 要： | 目的:观察三氧化二砷(As2O3)对NB4细胞珠端粒酶活性的影响,并探讨与细胞增殖、分化及细胞周期之间的关系。方法:应用MTT法、BNT法检测细胞的增殖抑制率、细胞分化率、流式细胞仪检测CD11b的表达率、细胞周期时相比率,以PCR为基础的TRAP法检测端粒酶活性。结果:As2O3能有效地使NB4细胞增殖抑制、诱导分化,伴随着端粒酶活性的变化为活性降低。细胞周期分析发现,G0/G1期高或S期低对应低水平端粒酶活性,G0/G1期低或S期高对应高水平端粒酶活性。结论:As2O3可抑制NB4细胞端粒酶活性,并与细胞分化、细胞周期时相有关。
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| 关 键 词： | 三氧化二砷 端粒酶 NB4细胞株 |
| 文章编号： | 1671-7554(2002)06-0565-02 |
| 修稿时间： | 2001-12-20 |
Effect of arsenic trioxide on telomerase activity in NB4 cells |
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| HAO Yun-liang,XU Cong-gao. Effect of arsenic trioxide on telomerase activity in NB4 cells[J]. Journal of Shandong University:Health Sciences, 2002, 40(6): 565-567 |
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| Authors: | HAO Yun-liang XU Cong-gao |
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| Affiliation: | Jining First People's Hospital |
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| Abstract: | Objective:To study the telomerase activity in NB4cells exposed to arsenic trioxide(As 2 O 3 ).Methods :The inhibition of cell proliferation,induction of cell differentiation,CD11b expression,mitotic cycle and telomerase activity were evaluated in NB4cells exposed to As 2 O 3 .Results:Telomerase activity was remarkably inhibited in NB4cells exposed to As 2 O 3 accompanied by inhibition of cell prolifera-tion,induction of cell differentiation and CD11b expression.A long G0/G1phase or a short S phase coincided with low telomerase activity while a short G0/G1phase or a long S phase coincided with high telomerase activity.Conclusion:As 2 O 3 can lower the telomerase activity in NB4cells,which has a close relationship with induction of cell differentiation and mitotic cycle. |
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| Keywords: | Arsenicalds Telomerase NB4cells |
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