Effect of stimulation of anterior
hypothalamic area on urinary bladder
function of the anesthetized rat |
| |
Authors: | I?Rocha Email author" target="_blank">L?Silva-CarvalhoEmail author K?M?Spyer |
| |
Institution: | (1) Instituto de Fisiologia, Faculdade de Medicina de Lisboa, Av. Prof. Egas Moniz , 1649-028 Lisbon, Portugal;(2) Dept. of Physiology, Royal Free and University College School of Medicine, London, UK |
| |
Abstract: | Abstract.
The hypothalamus is a
key area for the integration of the
autonomic features of affective behavior.
Hypothalamic defence area
(HDA) stimulation evokes major
cardiorespiratory changes as well
as modifications of general autonomic
activity both in the anesthetized
and conscious animal.
Micturition is due to an increase in
pelvic parasympathetic activity
and, in the cat, the anterior hypothalamus
has been implicated in
urinary bladder control with the
demonstration of a dorsolateral
vesicoconstrictor pathway and a
ventromedial inhibitory pathway.
In this study we have investigated
the effect of electrical and chemical
stimulation of the HDA on bladder
pressure and contractions in rat.
Female rats (n = 15) were anesthetized,
paralyzed and ventilated
artificially. Arterial blood pressure,
heart rate, urinary bladder pressure
and pelvic nerve activity were
recorded. HDA was electrically
(1 ms, 100 Hz, 5–10 s train at intensities
up to 150 µA) and chemically
(sodium glutamate, 50nl, 2mM)
stimulated. For statistical analysis
the t–test was used, data were expressed
as mean ± SEM. Values of t
were taken as significant when
p < 0.05.HDA stimulation at
100–150 µA evoked changes of
both mean blood pressure (mBP)
and bladder pressure (BlP). However,
stimulation at < 30µA allowed
a distinction within HDA of two
different regions, at the same antero–posterior and lateral level, but
separated 100–150µm in depth,
which evoked differential effects on
blood pressure and urinary bladder
pressure. Results show that low
intensity stimulation at ventral
sites evoked a significant increase
of mBP (from 102 ± 5.9 to
127 ± 8.6mmHg, n = 10, p < 0.0001)
with little changes of BlP (from
12 ± 2.2 to 16 ± 2.9cmH2O, n = 10,
p < 0.0005), whilst at more dorsal
sites significant increases of BlP
were elicited (from 12 ± 8.3 to
38 ± 4.6cmH2O,n = 10, p < 0.0001)
with only a small rise of mBP
(from 102 ± 6.2 to 111 ± 9.8mmHg,
n = 10, p < 0.005). Glutamate injections
at dorsal sites evoked a
rise of BlP (from 11 ± 2.2 to
30 ± 3.0cmH2O (n = 5; p < 0.0001)
with small changes in BP, whilst at
ventral sites (n = 4) glutamate microinjections
evoked changes in BP
but not of BlP. In conclusion stimulation
at different sites within HDA
can elicit separate changes in BP
and BlP. |
| |
Keywords: | hypothalamic
defence area HDA urinary
bladder urinary bladder pressure |
本文献已被 SpringerLink 等数据库收录! |
|