脊髓组织缺血再灌注早期自由基和超氧化物歧化酶的动态变化

目的：研究脊髓缺血再灌注早期脊髓组织丙二醛（MDA）含量及超氧化物歧化酶（SOD）活性变化，以探讨自由基在脊髓继发性损伤中的作用。方法：36只成年雄性SD大鼠采用腹主动脉肾下夹闭法造成脊髓缺血再灌注模型，连续观察再灌注后1h、4h、8h、16h、24h脊髓组织内MDA和SOD活性。结果：脊髓缺血再灌注后脊髓组织MDA立即上升，8h达最高峰，此后随之下降，但至24h仍高于正常对照组；SOD活性显著下降，8h达最低，随后逐渐回升，至24h基本接近缺血前。结论：MDA和SOD参与了脊髓缺血再灌注继发性损伤的病理过程。

Early dynic changes of free radical and superoxide diutas after spinal cord ischemia reperfusion

Objective: To investigate the early dynamic changes of free radical and superoxide dismutas(SOD) after spinal cord ischemia reperfusion associated with abdominal aortic cross-clamping and explore mechanism of ischemia reperfusion injury.Methods: 36 adult male Sprague-Dawley rats were divided randomly into control group and ischemia reperfusion groups.The concentration of malonyldialdehyed(MDA) and activities of SOD in spinal cord of these animals were measured at pre-ischemia,reperfusion 1 h,reperfusion 4 h,reperfusion 8 h,reperfusion 16 h and reperfusion 24 h,respectively.Results: The concentration of MDA increased immediately after reperfusion,reached to peak within 8 h and then began to decrease,but it still was higher than that of the control group.The activity of SOD decreased during reperfusion,the lowest point was present at 8 h and similar to that of the control group.Conclusion: It is suggested that free radical plays an important role in the secondary pathological injury of spinal cord induced by ischemia reperfusion.