双标记流式细胞术定量分析5-FU诱导胃癌细胞早期凋亡

目的 以Annexin V-FTTC/PI双标记流式细胞术检测5－FU诱导胃癌细胞株SCG7901早期凋亡。方法 250、125、50以及25mg/L不同浓度5－FU处理SCG7901胃癌细胞0、6、12、18、24、30h,Annexin V-FTTC/PI双标记流式细胞术检测肿瘤细胞凋亡。结果 早期（0－24h)瘤细胞凋亡比例随5－FU作用剂量增加和时间延长而显著提高（P＜0.01)；后期（24-30h)瘤细胞以坏死增加为主（P＜0.01)而凋亡比例反而降低。结论 5－FU诱导细胞凋亡呈时间以及剂量依赖关系。Annexin V/PI双标记法可以清楚区分正常、坏死、机械损伤以及凋亡细胞群落，是定量分析早期细胞凋亡的准确方法。

Quantitative analysis of early apoptosis of gastric tumor cells induced by 5-FU with Annexin V-FITC/PI double-labeled flow cytometry

Objective To quantify the early apoptosis of gastric cancer cell line SCG7901 induced by 5 FU flow cytometry with Annexin V FITC and PI double labeling. Methods The tumor cells were treated with 5 FU at concentration of 250, 125, 50, and 25 mg/L. The apoptosis was detected 0, 6, 12, 18, 24, 30 h after the treatment by using flow cytometry with Annexin V FITC and PI double labeling method. Results Early apoptosis percentage of tumor cells (0-24 h) was increased significantly with the affected time and the content of 5 FU. But it was decreased during 24-30 h after the treatment, while more necrotic cells were seen at same time. Conclusion 5 FU can induce early apoptosis of tumor cells in a time and dosage depended manner. Annexin V FITC/PI double labeling flow cytometry can distinguish dead cells from early apoptotic cells and be of great use in tumor cell apoptosis quantitative analysis.