热毒清滴眼液治疗单纯疱疹性角膜炎的实验研究

目的　评估中药热毒清滴眼液对兔实验性单纯疱疹性角膜炎 (herpessimplexkeratitis ,HSK)作用和超声雾化强化给药的疗效及急性毒性与眼的刺激反应。方法　 2 6只新西兰白兔 (5 2只眼 )角膜划痕接种I型单纯疱疹病毒 (herpessimplexvirus 1,HSV I)。HSK模型随机分为热毒清雾化组 :热毒清滴眼液通过超声雾化治疗 ;热毒清滴眼组 :热毒清滴眼液滴眼治疗 ;无环鸟苷组 :无环鸟苷滴眼液滴眼治疗 ;对照组 :生理盐水滴眼治疗。观察 18d时的疗效、对兔眼的刺激情况及对小鼠的不良反应。结果　三个治疗组有效率均显著高于对照组 (P <0 .0 5 ) ;热毒清雾化组有效率高于热毒清滴眼组和无环鸟苷组 ;热毒清滴眼液滴眼与无环鸟苷滴眼液滴眼疗效相同。对兔及小鼠都未发生全身和局部不良反应。结论　这个结果有力提示 ,中药热毒清滴眼液对HSK有明显疗效 ,超声雾化给药能强化药效。

Experimental study on herpes simplex keratitis treated with Reduqing eye drop

OBJECTIVE To evaluate the effect of herbal eye drop of Reduqing (clearing toxic heat) and potentialization of ultrasonic nebulous adiministration of Reduqing on experimental herpes simplex keratitis (HSK) in rabbits, the acute toxicity test and the irritating eye reaction. METHOD The 26 normal New Zealand White rabbits (52 eyes) were inoculated bilaterally with strain herpes simplex virus-1 (HSV-I) by corneal scarification. The models of the experimental HSK were randomly divided into four groups. The Reduqing nebulous group were treated with Reduqing eye drop by ultrasonic nebulization, the Reduqing eye group with Reduqing eye drop, the aciclovir eye drop with Aciclovir eye drop, and the control group were treated with normal saline eye drop. The effective rate of treatment within 18 days was recorded and the results were compared among the four groups. The irritating eye reaction in rabbits and the acute toxicity test in mice were observed.RESULTS The effective rates of three treatment groups were higher than that of the control group (P<0.05). The effective rate of Reduqing nebulous group was higher than that of the Reduqing eye drop group, while no significant difference was observed in the Reduqing eye drop group and acyclovir eye drop group. No general and local side effects occurred both in rabbits and mice. CONCLUSION The Reduqing eye drop has marked effect on experimental HSK in rabbits. The ultrasonic nebulous administration can improve the drug effect.