MMP-2和MMP-9蛋白在结肠癌中的表达

目的:分析基质金属蛋白酶-2(MMP-2)和MMP-9蛋白与结肠癌病理因素的关系,探讨MMP蛋白在结肠癌发生中的临床意义.方法:用SP免疫组化法检测31例结肠癌中MMP-2和MMP-9蛋白表达情况,并用SPSS10.0forWindows软件统计分析MMP蛋白与临床病理因素的关系.以20例溃疡性结肠炎、21例结肠腺瘤和10例正常结肠黏膜作为对照组.结果:MMP-2除结肠癌组和正常结肠黏膜组间相比具有显著性差异(10.0%vs54.8%,P<0.05)外,其余各组间比较差异无统计学意义,MMP-9各组间比较差异无统计学意义,从溃疡性结肠炎、结肠腺瘤到结肠癌中MMP-2和MMP-9蛋白表达阳性率不同且具有递增趋势.MMP-2和MMP-9蛋白表达与结肠癌的Duke’s分期及有无淋巴结转移显著相关(A B期:38.9%和27.8%;C D期:76.9%和84.6%;无淋巴结转移:38.9%和27.8%;有淋巴结转移:76.9%和84.6%,P<0.05).结论:MMP-2和MMP-9蛋白过度表达对结肠癌的诊断、Duke's分期及有无淋巴结转移判断具有重要的临床意义.

Expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 protein in colonic carcinoma

AIM: To analyze the correlation of matrix metallopro- teinase-2(MMP-2) and MMP-9 protein expression with the pathological factors in colonic carcinoma, and to investigate the clinical significance of matrix metallo- proteinases in the occurrence of colonic carcinoma. METHODS: The expression of MMP-2 and MMP-9 protein were measured by immunohistochemistry in the resected specimens from 31 patients with colonic carcinoma, and their relations with clinicopathologic factors were analyzed by SPSS 10.0 for Windows software. The specimens from ulcerative colitis (n = 20), colon adenoma (n = 21), and normal colon (n = 10) tissues were used as the controls. RESULTS: The positive rate of MMP-2 protein expres- sion was significantly different between colonic carcinoma and normal colon tissues (10.0% vs 54.8%, P <0.05), while no significant difference was found between other groups. The positive rates of MMP-2 and MMP-9 expression tended to increase in ulcerative colitis, colon adenoma, and colonic carcinoma gradually. The positive rates of MMP-2 and MMP-9 protein expression were significantly correlated withthe Dukes's staging and lymph node metastasis (C-D stage vs A-B stage: 76.9% vs 38.9%, P <0.05; 84.6% vs 27.8%, P <0.05; metastasis vs non-metastasis: 76.9% vs 38.9%, P <0.05; 84.6% vs 27.8%, P <0.05). CONCLUSION: Over-expression of MMP-2 and MMP-9 protein play important roles in the carcinogenesis and metastasis of colonic carcinoma.