EB病毒感染与中线恶性网织细胞增多症

使用一个针对ＥＢ病毒编码的小分子ＲＮＡ（ＥＢＥＲ）的寡核苷酸探针对１９例中线恶性网织细胞增多症病变组织进行了原位杂交检测，并配合免疫表型分析。结果为：（１）本组病例中１８／１９例（９４．７％）的病变组织中的异形淋巴样细胞表达Ｔ细胞分化抗原。ＣＤ３阳性１５例，ＵＣＨＬ１阳性９例，其中二者均阳性６例，但各例中的异形淋巴样细胞均未表达Ｂ细胞及组织细胞分化抗原；（２）ＥＢＥＲ原位杂交检测的阳性数高（１５／

Epstein-Barr virus infection in midline malignant reticulosis

n oligonucleotide probe directed against small RNAs encoded by EBV gene was used to detect EBV genome by in situ hybridization combined with immunophenotype analysis. The results showed that(1) in 18 of 19 cases(94.7%)atypical lymphoid cells(ALC)expressed T-cell differentiation antigen,15 cases were CD3 positive,9 cases were UCHL1 positive,6 cases were both CD3 and UCHL1 positive,no ALC gave positive reaction to B-cell and histiocvte markers.(2)15 of 19 cases gave positive reaction to EBER fsmall RNAs encoded by EBV gene)by in situ hybridization analysis and A LC were reactive to T-cell markers in all 15 cases but one. The resuIts of this study suggest that EBV infection is also present in midline malignant reticulosis, whlch is characterized by active T-cell proliferation. The role and effect of EBV infection in pathogenesis of lymphoproliferative idsorders was discussed.