骨巨细胞瘤的细胞遗传学研究

对１９例手术切除骨巨细胞瘤组织和４例骨巨细胞瘤细胞系进行了细胞遗传学研究，发现瘤细胞多为异倍体，染色体众数多为亚二倍体。核型分析表明第１７，１８号染色体丢失频率较高，染色体末端缺失和端粒融合是骨巨细胞瘤最常见的细胞遗传学改变。我们将骨巨细胞瘤染色体高度非随机断裂点和同一患者体细胞染色体脆性部位与目前公认的癌基因点进行比较，发现三个位点分别与癌基因ＭＥＴ，ＦＬＴ，ＹＥＳ２／ＰＤＧＦＢ（ｓｉｓ）同位或

Cytogenetic analyses on giant cell tumor of bone

er a 3 year period, 19 surgically resected giant cell tumors of boneand cell lines from 4 cases wereanalysed cytogenetically.The results indicate that most of the tumor cells are heteroploid,thechromosome model number is predominantly hypodiploid. Karyotypic analysis demonstrated that theloss of chromosome #17,#18 appears more frequently(with a loss rate of 60% and 45% respectively),tenninal deletion and telomeric fusion were the most common cytogenetic aberrations.We comparedhighly nonrandom tumor cell chromosome breakpoints and the same patient's somatic cell chromosomefragile sites with published oncogenes and found that 7q32,13q13, 22q12 were coincident with MET,FLT, YES2 / PDGFB (sis) respectively.Whether these loci abnonnalities correlate with tumorgenesisneeds further investigation.