胰腺癌的基因诊断

用ＰＣＲ－ＲＦＬＰ技术对２７例胰腺及其周围器官组织细针穿刺标本进行ｃ－Ｋｉ－ｒａｓ第１２密码子突变检测。结果１５例胰腺癌中１４例有ｃ－Ｋｉ－ｒａｓ第１２密码子突变。阳性率达９３．３％；而１１例慢性胰腺炎、胰腺囊肿、胆管炎、胆囊癌、壶腹癌及胃淋巴瘤均无ｃ－Ｋｉ－ｒａｓ第１２密码子突变。本研究结果证实ＰＣＲ－ＲＦＬＰ检测胰腺ｃ－Ｋｉ－ｒａｓ第１２密码子突变是诊断胰腺癌的一种快速、准确、敏感、实用的基因诊断方法。

Gene diagnosis of pancreatic adenocarcinoma

Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)technique was used to analyze c-Ki-ras codon l2 mutation in 27 fine needle aspiration biopsy(FNAB)specimens of the pancreas and its adjacent organs for the diagnosis of pancreatic adenocarcinoma.C-Ki-ras codon 12 mutation was present in 14 out of 15 cases of pancreatic adenocarcinoma,the positive rate was 93; 33%( 14/15); whereas no mutation was detected in those obtained from 12 patients with chronic pancreatitis, pancreatic cyst, gallbladder carcinoma,carcinoma of ampulla of Vater and gastric lymphoma. The results of this study verifies the PCR-RFLP technique as a practical,sensitive,rapid and reliable method for the detection of c-Ki-ras codon 12 mutation in the diagnosis of pancreatic adenocarcinoma.