黄芪多糖对高血压病血瘀证患者血清损伤的人脐静脉内皮细胞形态学、活性的影响

目的:观察黄芪多糖(astragalus polysaccharide,APS)对高血压病血瘀证患者血清损伤的人脐静脉内皮细胞(HUVEC-C)形态学、活性的影响。方法:将培养的高血压病血瘀证患者血清损伤的HUVEC-C分别用不同浓度的APS含药血清干预后,倒置显微镜下观察其形态学变化,MTT法检测其活性。结果:APS呈剂量依赖性升高损伤的细胞OD值,且随着药物组浓度的增加,细胞的形态趋向正常细胞。结论:APS能够抵抗高血压病血瘀证患者血清中有害因子对HUVEC-C的损伤作用,能增强细胞的活性和维持细胞的形态结构,并呈剂量依赖性。

Effects of Astragalus Polysaccharide on Activity and Morphological Changes in HUVEC Induced by Serum from Patients with Blood Stasis Syndrome and Hypertension

Objective: To observe the effects of Astragalus Polysaccharide (APS) on activity and morphological Changes in human umbilical vein endothelial cells (HUVEC) induced by serum from patients with blood stasis syndrome (BSS) and hypertension. Method: The HUVEC was affected by the serum of the patient with BSS associated with hypertension and different dosage of APS was applied for intervention.The activity of the HUVEC was determined by MTT assay and the morphological changes were observed by the inverse phase contrast microscope. Result: APS had the protective effect for lightening the HUVEC shape change affected by the serum from patient of BSS associated with hypertension and the activity was increased in a dose-dependent manner. Conclusion: APS can lighten the injury of the HUVEC affected by the serum from the patient of BSS associated with hypertension,increase cells activity and maintenance for the normal shape of the cells.