| 移植大鼠生精干细胞到裸鼠生精小管实验系统的建立 |
| |
| 引用本文： | 崔光辉,张超,郭新,秦洁,桂耀庭,蔡志明. 移植大鼠生精干细胞到裸鼠生精小管实验系统的建立[J]. 中国男科学杂志, 2009, 23(2) |
| |
| 作者姓名： | 崔光辉 张超 郭新 秦洁 桂耀庭 蔡志明 |
| |
| 作者单位： | 北京大学深圳医院男性生殖实验室,深圳,518036 |
| |
| 摘 要： | 目的 建立移植大鼠生精干细胞到裸鼠生精小管的实验系统.方法 采用一次腹腔注射busulfan 30mg/kg以消除裸鼠生精小管内源性生精细胞,制备生精干细胞移植受体小鼠;采用laminin粘附的方法分离大鼠生精干细胞;应用生精小管直接注射和输出管注射的方法移植分离到的大鼠生精干细胞进入受体裸鼠的生精小管内.结果 busulfan腹腔注射4周后受体裸鼠生精小管中的精子发生明显得到抑制.大鼠生精干细胞移植2～3个月后在受体裸鼠的生精小管中发现了大鼠的长型精子细胞.结论 成功建立了移植大鼠生精干细胞到受体裸鼠生精小管的试验系统.
|
| 关 键 词： | 干细胞移植 细精管 |
Experiment study on transplanting rat spermatogonial stem cell into nude mouse seminiferous tubules |
| |
| Cui Guanghui,Zhang Chao,Guo Xin,Qin Jie,Gui Yaoting,Cai Zhiming. Experiment study on transplanting rat spermatogonial stem cell into nude mouse seminiferous tubules[J]. Chinese Journal of Andrology, 2009, 23(2) |
| |
| Authors: | Cui Guanghui Zhang Chao Guo Xin Qin Jie Gui Yaoting Cai Zhiming |
| |
| Affiliation: | Cui Guanghui,Zhang Chao,Guo Xin,Qin Jie,Gui Yaoting,Cai Zhiming~* Laboratory of Male Reproduction,Shenzhen Hospital,Peking University,Shenzhen 518036,Guangdong China |
| |
| Abstract: | Objective To establish a experimental system of transplanting rat spermatogonial stem cell into mouse seminiferous tubules.Methods Recipient nude mice were treated with 30mg/kg busulfan(ip)to eliminate endogenous germ cells.Rat spermatogonial stem cells were isolated by Laminin adherence procedure.The isolated rat spermatogonial stem cells were transplanted into seminiferous tubules of recipient nude mice by direct injection and efferent injection.Results Four weeks after treatment of busulfan,endogenous ge... |
| |
| Keywords: | stem cell transplantation seminiferous tubules |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
