新型可降解聚合物聚乙二醇对苯二甲酸酯/聚对苯二甲酸丁二醇酯的细胞相容性研究

目的：研究新型可降解聚合物聚乙二醇对苯二甲酸酯／聚对苯二甲酸丁二醇酯(PEGT／PBT)的人脐静脉内皮细胞(HUVEC)相容性，对其在组织工程血管中的应用进行探讨。方法：对PEGT／PBT进行细胞毒性评价。观察并检测HUVEC在PEGB／PBT、Ⅰ型胶原改性(Col-)的PEGT／PBT、纤维连接蛋白改性(Fn-)的PEGT／PBT上的粘附和增殖，对细胞在粘附过程中的粘着斑蛋白进行免疫荧光染色观察。结果：PEGT／PBT细胞毒性不大于1级，能支持脐静脉内皮细胞的粘附和增殖。纤维连接蛋白和Ⅰ型胶原处理可促进HLIVEC在PEGT／PBT膜上的增殖，而且纤维连接蛋白可增加HUVEC在PEGT／PBT上20min、2h的粘附率，并促进细胞形成局部粘附结构。结论：新型可降解聚合物PEGT／PBT具有良好的血管细胞相容性，对其在组织工程血管中的应用值得进一步开发研究。

The Study on the Cytocompatibility of the PEGT/PBT Segmented Copolymers

Objective:To evaluate the cytocompatibility of biodegradable PEGT/PBT copolymers,which are intended to be used as scaffolds in vascular tissue engineering.Methods:The cytotoxicity of PEGT/PBT was evaluated through the determination of the L929 fibroblast cells' relative proliferation. The adhesion and proliferation of human umbilical vein endothelial cells were examined on PEGT/PBT,collagen coated PEGT/PBT and fibronectin coated PEGT/PBT membranes. Furthermore,the immunofluorescent detection of vinculin were conducted in HUVEC growed on the three kinds of PEG/PBT membranes. Results:The cytotoxicity of PEG/PBT had been graded not larger than grade 1. And PEGT/PBT supported HUVEC adhesion and proliferation well.The treatment with collagen and fibronectin enhanced the proliferation of HUVEC and had a statistical significance( P <0 05) compared to untreated PEGT/PBT.Moreover,fibronectin increased the adhesive ratio of HUVEC to a significant degree( P <0 05)at 20min and 2h respectively,and the fluorescent stainings of vinculin were more dense and extensive distributed on Fn coated PEGT/PBT at the time,indicating more focal adhesion formation.Conclusion:The experiment demonstrated the PEGT/PBT with qualified cytocompatibility,and had the potential for application in vascular tissue engineering.